微纳米图案化表面对细胞行为的影响

Effects of Micro/Nano Patterned Surfaces on Cell Behaviors

在再生医学领域中,材料对细胞生长和组织修复的调节作用一直是极为关键的问题。随着表面图案化技术的发展,在材料表面制备规则、可控、种类多样的图案化区域成为可能,因而该技术被广泛应用于再生医学、组织工程以及细胞诊断等相关学科领域。通过微纳米表面图案化方法,改变材料的化学性质和拓扑结构,从而实现对细胞粘附、迁移、增殖、凋亡、基因表达及分化的调控,受到越来越多关注。这其中涉及多种多样的微纳米结构、基底材料、修饰材料、细胞类型和细胞行为。该文归纳了目前主要的微纳米表面图案化方法,并从各向异性拓扑结构、各向同性拓扑结构以及复合型结构着手,概述了基于材料微纳米结构对细胞行为调控这一领域的研究工作,尝试总结细胞行为与生长环境的具体联系,为再生医学中所使用的支架、植入物的设计提供参考。

In the field of regenerative medicine,the regulation effect of materials on cell growth and tissue repair has always been a critical issue.With the development of surface patterning technique,it has become possible to prepare regular,controllable,and diverse patterned areas on the surface of materials.Therefore,patterning technque is widely used in related disciplines such as regenerative medicine,tissue engineering and cell diagnosis.Micro/nano surface patterning methods are used to change the chemical properties and topological structures of materials,so as to achieve the regulatory effect on cell adhesion,migration,proliferation,apoptosis,gene expression and cell differentiation,which have attracted more and more attention.This type of research is currently quite rich and diverse,involving a variety of micro/nano structures,substrate materials,modified materials,cell types and cell behavior.The micro/nano surface patterning methods mainly used in the current experiments are introduced in this article.Meanwhile,the research work in the field of material micro/nano structure regulation of cell behavior is also summarized from the aspects of anisotropic topological structure,isotropic topological structure and composite structure.Finally,the specific relationship between cell behavior and growth environment is summarized,so as to provide references for the design of scaffolds and implants used in regenerative medicine.