食源性创伤弧菌检测技术研究进展

Research Progress on Detection Technology of Foodborne Vibrio vulnificus

创伤弧菌是一种重要的海洋食源性病原菌,可经伤口或肠道感染多种水产养殖动物及人体。该菌在各种食源性病原体中病死率最高,发病迅速。因此,创伤弧菌的检测对食品安全以及患者诊断治疗十分重要。但传统检测方法存在步骤繁琐、耗时长等局限,限制了医学检验及食品安全检测的高效性。近年来,基于分子生物学和免疫学的迅速发展,以及纳米科学技术的兴起,国内外学者结合传统培养方法研究和建立了多种具有操作简便、准确高效、灵敏度高等特点的创伤弧菌快速检测技术,为创伤弧菌快速检测方法的进一步发展提供了新思路。该文对于微生物传统病原学检测技术、免疫学检测技术、分子生物学检测技术、生物芯片和生物传感技术等传统和新兴的创伤弧菌检测手段进行了总结,以期为食源性创伤弧菌的有效防控及患者的诊断治疗提供科学依据。

Vibrio vulnificus(V.vulnificus)is an important marine foodborne pathogen,which could infect a variety of aquaculture animals and humans via a wound or intestinal infection.Moreover,this bacteria have the highest mortality among various foodborne pathogens,occurring rapidly.Therefore,the detection of V.vulnificus is very significant for food safety and the diagnosis of patients.However,traditional detection methods still have some limitations,such as cumbersome steps and long time-consuming.Meanwhile,they also limit the accuracy of medical inspection and food safety detection.In recent years,based on the rapid development of molecular biology and immunology,as well as the rise of nano science and technology,domestic and foreign scholars have studied and established varieties of rapid detection techniques for V.vulnificus,with the characteristics of simple operation,accuracy,high efficiency and high sensitivity,providing a new idea for the further development of rapid detection methods of V.vulnificus.Thus,this paper reviews and summarizes the traditional and emerging detection methods of V.vulnificus,such as microbial traditional pathogenic detection technique,immunological detection technique,molecular biology detection technique,biochip and biosensor technique,in order to provide a scientific basis for the effective prevention and control of V.vulnificus,as well as the diagnosis and treatment of patients.