不同水解方式下蚕丝丝素蛋白材料的止血性能

Hemostasis properties of silk fibroin materials under different types of hydrolysis

为提升蚕丝丝素蛋白材料的止血性能,采用盐解和酶解2种方式水解蚕丝丝素蛋白,通过冷冻干燥法制备得到2种蚕丝丝素蛋白材料。通过凝胶过滤色谱、水溶性实验、凝血因子实验、血小板黏附实验、酶联免疫吸附实验等分析了2种蚕丝丝素蛋白材料的分子质量、水溶性和止血效果。结果表明:与盐解法相比,酶解所得材料具有较低的分子质量和较好的水溶性,有利于激活凝血因子Ⅻ从而对内源性凝血起到促进作用,止血时血小板黏附量和血小板第4因子含量会明显增加,大鼠肝部出血量减少且出血时间缩短,其止血效果优于市售云南白药。

Aiming at the improvement of hemostasis properties of silk fibroin materials fabricated by salt hydrolysis and enzyme hydrolysis method,the silk fibroin materials were prepared via a freeze-drying method.The molecular weight,water solubility and hemostatic effect of the resultant material were analyzed by gel filtration chromatography,water solubility test,coagulation factor test,platelet adhesion test,enzyme-linked immunosorbent assay and rat liver injury model.The results showed that compared with the salt hydrolysis method,the enzyme hydrolysis method could endow a lower molecular weight and better water soluble,which is conducive to activating the coagulation factor XII to promote endogenous coagulation.Moreover,the platelet adhesion and platelet factor 4 content increase significantly,the amount of bleeding from the rat liver is reduced with shortened bleeding time,indicating better hemostatic effect than that of Yunnan white medicine.