五味子多糖的分离、纯化及结构表征

Isolation,purification and characterization of polysaccharide from Schisandra chinensis

[目的]通过对五味子多糖的分离纯化、结构表征,以期为五味子的进一步开发利用提供科学研究数据。[方法]采用水提醇沉法得五味子粗多糖,通过分级醇沉及葡聚糖凝胶G-150进一步分离纯化得五味子多糖组分。采用苯酚-硫酸法测定多糖的含量,高效分子排阻色谱联用多角度激光光散射检测器和示差检测器(HPSEC-MALLS-RID)的方法测定多糖的分子量,1-苯基-3-甲基-5-吡唑啉酮(PMP)试剂柱前衍生结合高效液相色谱(HPLC)法测定多糖的单糖组成,红外光谱(IR)和核磁共振波谱(NMR)分析多糖的结构。[结果]分离纯化得五味子多糖组分SCP-3-1,其多糖含量为74.28%,分子量为84.9 kDa,主要由甘露糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖(摩尔比为0.09∶0.19∶19.82∶2.08∶0.13)组成。IR及NMR数据分析表明是一种以α-糖苷键连接的吡喃型多糖。[结论]本研究对于进一步解析多糖结构具有一定的借鉴意义和参考价值,也为五味子的进一步应用提供了科学依据。

[Objective]The research on the isolation and characterization of polysaccharide from Schisandra chinensis is expected to provide scientific data for the further study of Schisandra chinensis.[Methods]The crude polysaccharides of Schisandra chinensis were obtained by water extraction and ethanol precipitation.A polysaccharide from Schisandra chinensis was further isolated and purified by graded ethanol precipitation and Sephadex G-150.Phenol-sulfuric acid method was used to determine the content of polysaccharide.High performance size exclusion chromatography coupled with multi-angle laser light scattering detector and refractive index detector method(HPSEC-MALLS-RID),high performance liquid chromatography(HPLC),infrared spectroscopy(IR)and nuclear magnetic resonance(NMR)were used to determine its molecular weight,monosaccharide compositions,functional groups and structure,respectively.[Results]A polysaccharide from Schisandra chinensis(SCP-3-1)was isolated from Schisandra chinensis.SCP-3-1 was mainly composed of mannose,galacturonic acid,glucose,galactose and arabinose in molar ratios of 0.09∶0.19∶19.82∶2.08∶0.13 with polysaccharide content of 74.28% and molecular weight of 84.9 kDa.IR and NMR analyses indicated that it was a pyran polysaccharide connected with α-glycosidic bonds.[Conclusion]This study can provide useful reference for further analyzing structure of polysaccharide,and also provide a scientific basis for the application of Schisandra chinensis.