碳点用于荧光成像介导的原位膀胱癌靶向光动力/光热治疗

Orthotopic Bladder Tumor Targeted Carbon Dots for Fluorescence Imaging-guided Phototherapy

膀胱癌是泌尿系统中最常见的肿瘤之一。目前,化疗与手术切除等治疗手段不能有效治愈该肿瘤,并且上述两种治疗方式会产生副作用,降低患者的生活质量。光疗则为膀胱癌提供了一种新的治疗手段。在本工作中,以靶向膀胱癌细胞的小肽PLZ4修饰多功能碳点,得到了兼具靶向膀胱癌细胞及光动力/光热治疗功能的PLZ-4碳点(PCDs)。细胞实验表明该PCDs能够用于膀胱癌细胞靶向成像,并且在635 nm激光照射下,能高效杀灭膀胱癌细胞。活体实验表明经过尾静脉注射后,PCDs能够有效地聚集在原位膀胱癌处,在635 nm激光照射下,原位膀胱癌能够被完全清除,表明该PCDs在荧光成像介导的光动力/光热治疗原位膀胱癌中有潜在的应用价值。

Bladder cancer has become one of the most frequent malignant tumors in the urinary system.Chemotherapy and radical cystectomy could not effectively cure this illness and even lead to side effects and low-quality life,which seriously threaten the lives of patients.Phototherapy provides a new method for bladder cancer treatment.Here,multifunctional carbon dots theranostics was developed and modified by a bladder cancer-specific peptide(PLZ4).PLZ4-carbon dots(PCDs)integrate targeted delivery,photodynamic therapy(PDT),and photothermal therapy(PTT)in one procedure.In vitro fluorescence imaging revealed that PCDs tend to aggregate in tumor cells than in normal cells,thus allowing them to effectively ablate MB-49 bladder cancer cells under a 635 nm laser.In vivo biodistribution showed that the PCDs specifically accumulated in bladder cancer tissues but exhibited negligible fluorescence in normal bladder tissues.After combined PDT/PTT,the orthotopic bladder tumor can be completely killed,thereby proving that PCDs have a potential as a candidate for bladder cancer treatment.