黄地老虎幼虫取食诱导的棉花植株挥发物驱避雌蛾产卵

Cotton plant volatiles induced by larval feeding of Agrotis segetum(Lepidoptera:Noctuidae)deter oviposition of conspecific females

【目的】评价黄地老虎Agrotis segetum幼虫取食危害诱导棉花植株产生的虫害诱导植物挥发物(herbivore-induced plant volatiles,HIPVs)对雌蛾产卵选择的影响,并鉴定HIPVs的主要组分及其功能,以筛选对雌蛾产卵有吸引或驱避作用的活性成分。【方法】利用气相色谱-质谱联用仪(GC-MS)筛选出棉花植株被黄地老虎幼虫取食危害诱导产生的HIPVs,然后利用触角电位仪(EAG)和产卵笼分别测试黄地老虎雌蛾对HIPVs主要组分的电生理反应和产卵选择。【结果】黄地老虎雌蛾显著偏好在健康棉株上产卵,产卵量为在受幼虫危害棉株上的6.59倍。黄地老虎幼虫取食棉花植株诱导产生的HIPVs主要包括苯甲醛、芳樟醇、β-石竹烯、葎草烯、十四烷、十六烷等,其中黄地老虎雌蛾对苯甲醛和芳樟醇的触角反应较明显,并且随苯甲醛和芳樟醇浓度增加而逐步增大。在产卵笼试验中,芳樟醇可显著驱避黄地老虎雌蛾产卵,而苯甲醛则吸引其产卵。【结论】黄地老虎幼虫危害棉花植株产生的HIPVs对雌蛾产卵具有明显驱避作用,其中芳樟醇有望作为一种产卵驱避剂。

【Aim】The oviposition deterrence of herbivore-induced plant volatiles(HIPVs)from cotton plants damaged by the larvae of the turnip moth,Agrotis segetum,was evaluated on conspecific females,and the composition and functions of the major HIPVs were characterized in this study in order to identify the active components attracting or deterring adult oviposition.【Methods】We identified HIPVs from cotton plants damaged by A.segetum larvae using a gas chromatography-mass spectrometer(GC-MS)and evaluated the electrophysiological activity and oviposition deterrent bioactivity of the major HIPVs to female adults of A.segetum using the electroantennogram(EAG)and oviposition cage bioassay method,respectively.【Results】The female adults of A.segetum preferred to lay eggs on the healthy cotton plants over the damaged cotton plants,and the number of eggs laid on the healthy cotton plants was 6.59 times as high as that on the damaged cotton plants by A.segetum larvae.The HIPVs emitted from the cotton plants damaged by A.segetum larvae mainly consisted of benzaldehyde,linalool,β-caryophyllene,humulene,tetradecane,hexadecane,etc.Among them,benzaldehyde and linalool elicited strong EAG responses on the female antennae,showing a dose-response pattern.In the oviposition cage bioassay,linalool significantly deterred oviposition of female adults of A.segetum,while benzaldehyde attracted female adults to oviposit.【Conclusion】HIPVs emitted from cotton plants damaged by A.segetum larvae significantly deter oviposition of conspecific females,and linalool is considered as a potential oviposition deterrent.