抗菌肽SK_(66)的生物信息学分析

The Bioinformatics Analysis of the Antibacterial Peptide SK_(66)

从数据库中检索出CG13551基因氨基酸序列信息,生理生化分析显示:SK_(66)蛋白含有107个氨基酸残基,分子量约11948.3Da,理论等电点为8.96。该蛋白中,甘氨酸(Gly)含量最高,为14.0%;其次为谷氨酸(Glu)和丙氨酸(Ala),分别为11.2%和9.3%。SK_(66)蛋白是一个亲水蛋白质但不是一个分泌蛋白。SK_(66)蛋白不存在跨膜结构域。蛋白质二级结构包括57.96%α-螺旋、10.28%β-折叠、β-转角以及31.78%无规则卷曲等构象。进化树显示,主要分成两类,较为高等的动物分为第一类,包括人、小鼠、河豚;而较为低等的动物归为一类,包括斑马鱼、果蝇。这与物种的进化程度相一致。

The amino acid sequence information of CG13551 gene is retrieved from the database.Physicochemica l analysis show that the SK_(66)protein contains 107 amino acid residues,with a molecular weight of 11948.3 Da and a theoretical isoelectric point of 8.96,the content of glycine(Gly)is 14.0%.Glutamic acid(Glu)and alanine(Ala)are 11.2%and 9.3%,respectively,SK_(66)protein is a hydrophilic protein and is not a secreted protein.SK_(66)protein does not have transmembrane domain.Protein secondary structures include 57.96%α-helix,10.28%β-folding,β-turning and 31.78%random crimp.The evolutionary tree is mainly divided into two groups.The higher animals are divided into the first group,including human,mouse and puffer fish,while the lower animals are divided into the first group,including zebrafish and fruit fly.This is consistent with the degree of evolution of the species.