一测多评法测定不同产地巴戟天中5种蒽醌类成分的含量

Determination of Five Anthraquinones in Morinda Officinalis from Different Producing Areas by QAMS

利用高效液相色谱法,建立同时测定巴戟天中5种蒽醌类成分含量的一测多评法。采用Venusil MP C18色谱柱(4.6 mm×250 mm,5μm),流动相:乙腈(A)-0.3%磷酸溶液(B),梯度洗脱(0~40 min,38%A,40~50 min,38%A→55%A;50~70 min,55%A→60%A),流速:0.8 mL/min,柱温:30℃,进样量:10μL,检测波长:266 nm,以2-羟基-1-甲氧基蒽醌为内参物,建立巴戟天中其他蒽醌类成分与2-羟基-1-甲氧基蒽醌之间的相对校正因子,并计算其含量。结果显示,相对校正因子具有良好的耐用性,相对标准偏差(relative standard deviation,RSD)值均小于2.00%;一测多评法与外标法所测结果无明显差异,RSD值均小于3.00%。

A method for the determination of five Anthraquinones in Morinda officinalis is established by high performance liquid chromatography(HPLC).The chromatographic separation is performed on Venusil MP C18 column(4.6 mm×250 mm,5μm)with the mobile phases consisting of acetonitrile(A)-0.3%phosphoric acid(B)and gradient elution(0~40 min,38%A,40~50 min,38%A→55%A;50~70 min,55%A→60%A)at the flow rate of 0.8 mL/min.The column temperature,injection volume and detection wavelength are set at 30℃,10μL and 266 nm,respectively.HPLC is used to establish the relative correction factors of other components with the reference substance 1-methoxy-2-hydroxy anthraquinonoid.The results show that the relative correction factor had good durability and relative standard deviation(RSD)values are all less than 2.00%.There is no significant difference between the QAMS method and the external standard method,and the RSD values are all less than 3.00%.