猪伪狂犬病病毒GD株的分离鉴定及致病性研究

Isolation, Identification and Pathogenicity of porcine pseudorabies virus GD strain

从广东某猪场病死猪中分离到一株疑似猪伪狂犬病病毒(PRV)的毒株,该病毒在猪睾丸细胞上出现细胞聚集、脱落、拉网等典型的细胞病变,在猪睾丸细胞上测得其TCID50值为106.8/0.1 mL。PCR反应可以扩增出特异性370 bp DNA片段,gE基因测序结果与GenBank中收录的多株国内外PRV毒株进行比对分析,同源性介于97.6%~99.9%之间。证实分离的病毒株为伪狂犬病病毒,并命名为PRV GD株。将分离所得的PRV GD株接种小鼠、兔、仔猪和育肥猪,均能使之发病并出现不同程度死亡,该毒株表现出较强的致病性。

A virus suspected to be porcine pseudorabies virus(PRV)was isolated from dead pigs in Guangdong province. The typical cytopathic effect(CPE)could be observed when the isolated virus was cultured on the swine testicular cells and it’s tissue culture infectious dose(TCID50)was 106.8/0.1 mL. A specific 370 bp long DNA fragment could be expanded using polymerase chain reaction(PCR)analysis from the isolated virus DNA. The sequence analysis results showed that the nucleotide identity of gE-gene of PRV GD strain were between 97.6% ~99.9%comparing with several domestic and foreign PRV strains published in GenBank. The isolated virus strain was confirmed to be porcine pseudorabies virus and named as PRV GD strain. Inoculation of mice、rabbits、piglets and fattening pigs can cause the disease and death with strong pathogenicity.