干热环境和噪音刺激对大鼠结肠组织和血清代谢的影响

Effects of dry-hot environment and noise stimulation on the colon tissue and serum metabolism in the rats

目的探讨干热环境和噪音刺激对大鼠结肠组织和血清代谢的影响,为预防不良环境对机体的不利影响提供实验依据。方法25只无特定病原体(SPF)级Wistar大鼠随机分为正常组(10只)和实验组(15只),其中实验组大鼠用干热环境、干热药物、干热性饮水及噪音等方法进行刺激,刺激10 d后比较2组大鼠的体重、结肠组织病理学和超微结构变化;用核磁共振氢谱技术检测两组大鼠血清中的差异代谢组分。结果与正常组比较,实验组大鼠体重降低,差异有统计学意义(P<0.05);实验组大鼠结肠组织存在组织病理学和超微结构的改变;实验组大鼠血清中低密度脂蛋白(LDL)、异亮氨酸、亮氨酸等代谢组分水平发生显著性改变,与对照组比较差异有统计学意义(P<0.05)。结论干热环境和噪音刺激引起大鼠结肠组织形态学异常,产生炎症;干热环境和噪音刺激会导致大鼠机体内的糖、脂、蛋白和能量代谢的紊乱。

Objective To investigate the effects of chronic stimulation such as dry-hot environment,dry-hot diet and noise on the colon tissue and serum metabolism in the rats,so as to provide experimental basis for preventing the adverse effects of bad environment on the human body.Methods A total of 25 specific pathogen free(SPF)Wistar rats were randomly divided into the normal group(n=10)and the experimental group(n=15).Among them,the rats in the experimental group were stimulated with dry-hot environment,dry-hot medicine,dry-hot drinking water and noise.The changes of body weight,colon histopathology and ultrastructure were compared between the two groups after ten days of the stimulation.Nuclear magnetic resonance hydrogen spectroscopy(1H-NMR)was used to detect the differential metabolic components in serum of the two groups.Results Compared with that of the normal group,the weight of rats in the experimental group decreased and the difference was statistically significant(P<0.05).The histopathological and ultrastructural changes of the colon tissue were observed in the experimental group.The serum levels of low density lipoprotein(LDL),isoleucine,leucine and other metabolites in the experimental group were significantly changed,the differences were statistically significant(P<0.05).Conclusion Dry-hot environment and noise stimulation will induce abnormal colon histomorphology and inflammatory response in the rats.Dry-hot environment and noise stimulation can disturb the metabolism of sugar,fat,protein and energy in the rats.