Pib、Pi9、Pi2、Pi54和Pish在粳稻品种(系)中的分布及对穗颈瘟的抗性

Distribution of Pib,Pi9,Pi2,Pi54 and Pish genes in Japonica rice varieties(lines)and resistance to panicle blast

【目的】探明抗稻瘟病基因Pib、Pi9、Pi2、Pi54和Pish在粳稻品种(系)中的分布情况及不同基因型对稻瘟病的抗性水平。【方法】利用该5个基因的分子标记及人工接种稻瘟病的方法对93份材料进行研究。【结果】包含3个、2个和1个抗性基因的材料分别为19份(占20.4%)、25份(占26.9%)和28份(占30.1%),但这5个抗性基因在93份材料中均有分布。携带抗性基因Pib、Pi9、Pi2、Pi54和Pish的材料分别为49份(52.7%)、16份(17.2%)、8份(8.6%)、31份(33.3%)、31份(33.3%),抗性基因Pib的检出率最高;抗性较好的6个基因型或基因组合分别是Pi9、Pi54、Pib+Pi9、Pib+Pi2、Pib+Pish和Pi9+Pi54,但检出率均很低。【结论】本研究进行的抗稻瘟病基因检测和抗病性评价结果为稻瘟病抗性育种提供了可靠的理论依据。

【Objective】The present paper aimed to clarify the distribution of rice blast resistance genes Pib,Pi9,Pi2,Pi54 and Pish in Japonica rice varieties(lines)and the resistance levels of different genotype to rice blast.【Method】A total of 93 rice materials were assessed by artificial inoculation and screening of molecular markers tightly linked to 5 major resistance genes.【Result】Three,two and one resistance genes were found in 19(20.4%),25(26.9%)and 28(30.1%)materials,respectively,and five resistance genes corresponding to markers were found in all varieties(lines).There were 49(52.7%),16(17.2%),8(8.6%),31(33.3%)and 31 varieties(lines)(33.3%)containing rice blast resistance gene Pib,Pi9,Pi2,Pi54 and Pish in 93 varieties(lines),and the genetic frequency of Pib was the highest within this collection.A total of six genotypes and gene combinations(Pi9,Pi54,Pib+Pi9,Pib+Pi2,Pib+Pish and Pi9+Pi54)are applicable in the rice-breeding,due to the resistance to rice blast but with low distribution in the tested varieties(lines).【Conclution】The study provided a scientific basis for breeding rice cultivars with rice blast-resistance.