野生香菇黔香筛5号菌丝的生物学特性及抗氧化活性

Biological characteristics and antioxidant activities of wild Lentinula edodes

【目的】探明香菇黔香筛5号菌丝的生物学特性及不同添加物发酵对菌丝体中香菇多糖抗氧化活性的影响,为高活力菌种的制备及功能性产品开发奠定基础。【方法】采用固体和液体培养方式,以菌落直径和菌丝体干重作为主要考察指标,结合培养过程中菌丝的生长性状,研究香菇黔香筛5号菌丝的生物学特性及在不同碳氮源、培养温度、初始pH等条件下的生长情况;测定该菌株经添加皇竹草(HLNT)和中药渣(ZLNT)浸煮汁液体发酵后其香菇多糖(LNT)对DPPH、ABTS及羟基自由基的清除作用。【结果】母种培养基的最佳碳氮源为葡萄糖和硫酸铵,最适培养温度25~30℃,初始pH 4.0~6.0;菌丝体生长液体培养基的最适碳源为蔗糖,氮源为硫酸铵或蛋白胨,培养温度25℃,接种量3%(V/V),pH 6.0。ZLNT对DPPH和羟基自由基具有显著清除作用,当浓度为5 mg/mL时,清除率分别达86.7%和73.5%;HLNT对ABTS自由基清除效果较好,浓度为5 mg/mL时的清除率为75.5%。【结论】初步筛选出适宜于香菇黔香筛5号菌丝生长的营养需求及培养条件,该菌株表现出较好的副产物适料性,添加浸煮汁可促进发酵物抗氧化活性。

【Objective】The present paper aimed to explore the biological characteristics of a wild Lentinula edodes and the fermentation of different additives on the antioxidant activity of lentinan polysaccharide in mycelium, and to lay the foundation for the preparation of high vitality strains and the development of functional products. 【Method】Colony diameter and dry weight of mycelium were used as main indexes, the biological traits and growth of mycelium of tested strains under different carbon and nitrogen sources, culture temperature and initial pH were studied by solid and liquid culture. Meanwhile, the scavenging effects of Lentinan on DPPH,ABTS and hydroxyl radicals were further determined by adding fermented liquid Huangzhu grass(HLNT) and Chinese medicine residue(ZLNT). 【Result】The best carbon and nitrogen sources in the solid medium were glucose and ammonium sulfate, and the optimum culture temperature was 25-30 ℃, initial pH 4.0-6.0. When sucrose was used as carbon source, ammonium sulfate or peptone as nitrogen source, mycelium biomass was accumulated in liquid medium. The culture temperature 25 ℃, inoculation amount 3%(v/v) and pH 6.0 were suitable conditions. ZLNT had significant scavenging effect on DPPH and hydroxyl radicals, as the concentration was 5 mg/mL, the scavenging rate was 86.7% and 73.5%, respectively. The scavenging rate of HLNT with 5 mg/mL concentration to ABTS radical was 75.5%. 【Conclusion】The nutritional requirements and cultivation conditions suitable for mycelia growth of the wild L.edodes were preliminarily screened, and the strain showed better suitability for byproducts, which antioxidant activity of the liquid fermentation could be promoted by adding the byproduct immersion solution.