基于Wnt/β-catenin信号通路探讨miR-613对宫颈癌SiHa细胞增殖、迁移与侵袭的影响

Investigate the Effects of miR-613 on the Proliferation, Migration and Invasion of Cervical Cancer SiHa Cells Based on Wnt/β-catenin Signaling Pathway

目的:研究基于Wnt/β-catenin信号通路探讨微小RNA(mi R)-613对宫颈癌Si Ha细胞增殖、迁移与侵袭的影响。方法:体外培养宫颈癌Si Ha细胞和正常宫颈上皮细胞H8,检测细胞中mi R-613表达。根据转染mi R-613 mimic浓度不同,将Si Ha细胞分为0μmol/L组,100μmol/L和200μmol/L组。MTT法检测细胞增殖情况,划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力。蛋白免疫印迹法检测mi R-613表达对Wnt/β-catenin信号通路蛋白β-catenin、Vimentin、E-cadherin和MMP9表达的影响。结果:宫颈癌Si Ha细胞中mi R-613表达水平均显著低于正常宫颈上皮细胞H8,差异有统计学意义(P<0.05)。转染mi R-613mimic后,100μmol/L、200μmol/L组Si Ha细胞中mi R-613表达水平显著上调,并且具有浓度依赖性(P<0.05)。与0μmol/L组相比,100μmol/L、200μmol/L组的Si Ha细胞增殖,迁移,侵袭能力均明显下降,并且具有浓度依赖性(P<0.05)。免疫印迹结果,与0μmol/L组相比,100μmol/L、200μmol/L各浓度组的Si Ha细胞Wnt/β-catenin信号通路蛋白β-catenin、Vimentin和MMP9表达显著下调,E-cadherin表达显著上调,并且具有浓度依赖性(P<0.05)。结论:mi R-613能通过抑制Wnt/β-catenin信号通路抑制人宫颈癌细胞系Si Ha细胞的增殖,迁移和侵袭。

Objective: To study the effect of microRNA(mi R)-613 on the proliferation, migration and invasion of cervical cancer Si Ha cells based on the Wnt/β-catenin signaling pathway. Methods: Cervical cancer Si Ha cells and normal cervical epithelial cells H8were cultured in vitro, and the expression of mi R-613 in the cells was detected. According to the different concentrations of transfected mi R-613 mimic, Si Ha cells were divided into 0 μmol/L group, 100 μmol/L and 200 μmol/L group. Cell proliferation was detected by MTT assay, cell migration ability was detected by scratch assay, and cell invasion ability was detected by Transwell assay. Western blotting was used to detect the effects of mi R-613 expression on the expression of Wnt/β-catenin signaling pathway proteins β-catenin, Vimentin, E-cadherin and MMP9. Results: The expression level of mi R-613 in human cervical cancer cells Si Ha was significantly lower than that in normal cervical epithelial cells H8, and the difference was statistically significant(P<0.05). After transfection of mi R-613mimic, the expression levels of mi R-613 of Si Ha cells in the 100 μmol/L and 200 μmol/L concentration groups were significantly up-regulated, and it was concentration-dependent(P<0.05). Compared with the 0 μmol/L group, the Si Ha cells in the 100 μmol/L and 200μmol/L concentration groups had significantly lower proliferation, migration and invasion abilities, and it was concentration-dependent(P<0.05). Western blot results showed that compared with the 0 μmol/L group, the expressions of Wnt/β-catenin signaling pathway proteins β-catenin, Vimentin and MMP9 were significantly down-regulated, and the expression of E-cadherin was significantly up-regulated in Si Ha cells in the 100 μmol/L and 200 μmol/L concentration groups, and they were concentration-dependent(P<0.05). Conclusion:mi R-613 can inhibit the proliferation, migration and invasion of human cervical cancer cell line Si Ha cells by inhibiting the Wnt/β-catenin signaling pathway.