老年缺血性心力衰竭的心脏DNA甲基化编码重编程与心肌细胞焦亡、铁死亡的关联

The Relationship between Cardiac DNA Methylation Code Reprogramming and Myocardial Cell Pyrolysis and Ferroptosis in Elderly Ischemic Heart Failure

目的:探讨老年缺血性心力衰竭的心脏DNA甲基化编码重编程与心肌细胞焦亡、铁死亡的关联性。方法:2019年12月到2021月2月,选择在本院诊治的老年缺血性心力衰竭115例作为心衰组,同期选择在本院体检的非心血管疾病老年人群115例作为对照组。检测心脏DNA甲基化编码重编程、心肌细胞焦亡、铁死亡指标表达情况并进行相关性分析。结果:心衰组的心脏DNA甲基化编码重编程指标-mi R-92a、mi R-130a相对表达水平高于对照组(P<0.05)。心衰组的Caspase-1蛋白、Caspase-4蛋白相对表达水平高于对照组(P<0.05)。心衰组的铁调素含量高于对照组(P<0.05)。在两组230例入选者中,Spearsman相关分析显示:缺血性心力衰竭与mi R-92a、mi R-130a、半胱氨酸蛋白酶1(Caspase-1)、半胱氨酸蛋白酶4(Caspase-4)、铁调素存在正向相关性(P<0.05)。Logistic回归分析显示:mi R-92a、mi R-130a、Caspase-1、Caspase-4、铁调素为导致缺血性心力衰竭发生的重要因素(P<0.05)。结论:老年缺血性心力衰竭患者多伴随有心脏DNA甲基化编码重编程与心肌细胞焦亡、铁死亡,后三者与缺血性心力衰竭的发生存在关联性,也是导致缺血性心力衰竭发生的重要因素。

Objective:To investigate the relationship between cardiac DNA methylation code reprogramming and myocardial cell pyrolysis and ferroptosis in elderly ischemic heart failure.Methods:From December 2019 to February 2021,115 cases of elderly patients with ischemic heart failure diagnosed and treated in the Department of Cardiology of our hospital were selected as the heart failure group,and 115 cases of elderly patients with non-cardiovascular diseases who were in the hospital for physical examination during the same period were selected as the control group.Detected of cardiac DNA methylation coding reprogramming,cardiomyocyte pyrolysis,iron death index expression all the two groups and given correlation analysis.Results:The relative expression levels of cardiac DNA methylation encoding reprogramming indicators-miR-92a and mi R-130a in the heart failure group were higher than those in the control group(P<0.05).The relative expression levels of Caspase-1 protein and Caspase-4 protein in the heart failure group were higher than those in the control group(P<0.05).The content of hepcidin in the heart failure group were higher than that in the control group(P<0.05).In the two groups of 230 candidates,Spearsman correlation analysis showed that there were positive correlation between ischemic heart failure and mi R-92a,mi R-130a,Caspase-1,Caspase-4,hepcidin(P<0.05).Logistic regression analysis showed that mi R-92a,mi R-130a,Caspase-1,Caspase-4,hepcidin were important factors leaded to ischemic heart failure(P<0.05).Conclusion:Elderly patients with ischemic heart failure are mostly accompanied by cardiac DNA methylation coding reprogramming,myocardial cell pyrolysis and iron death.The latter three are related to the occurrence of ischemic heart failure and also lead to ischemic heart failure.An important factor in the occurrence of failure.