摘要
大珠母贝主要用于生产高价值的金银色有核珍珠。通过对三亚大珠母贝种群的进行取样,我们培育了一个外套膜边缘具有金色珍珠层的珍珠贝品系。通过对总类胡萝卜进行测定,发现该品系外套膜组织中的总类胡萝卜素含量显著高于对照组。我们筛选了两个品系之间的差异表达基因。有28285个基因在转录组中被注释。与对照组相比,共有295个上调基因和254个下调基因在选育系中被注释。与脂质运输和代谢相关的基因,如脂肪酸结合蛋白、细胞色素P4503A和ABC1基因上调。候选基因在选育系中的表达水平高于对照组。这些发现有助于了解大珠母贝金唇品系中类胡萝卜素的代谢和黄色珍珠层的形成机制。
Pearl oyster Pinctada maxima is mainly used to produce high-valued gold and silver colored nucleated pearls.We developed a line with golden nacre in the mantle edge by sampling breeders from the Sanya population of pearl oyster P.maxima.It is found that the total carotenoid content(TCC)in the mantle tissues of the strain group(SG)was significantly higher than that of the control group(CG).Differentially expressed genes between the strain and control group(a line with silver nacre in the mantle edge)were screened.A total of 28285 genes were annotated in the transcriptome.Totally,295 up-regulated genes and 254 down-regulated genes were annotated which the strain compared with the CG.Lipid-transport and metabolism genes,such as fatty acid-binding protein,cytochrome P4503A and ATP-binding cassette sub-family A were up-regulated in the line.The expression levels of candidate genes in the line were higher than those in the CG,which are basically consistent with the transcriptome results.These findings are helpful to understand carotenoid metabolism in golden colored nacre formation of pearl oyster P.maxima.
作者
赵子涵
龚舜莲
王姿曼
雷超
李俊辉
郑哲
杨创业
邓岳文
ZHAO Zihan;GONG Shunlian;WANG Ziman;LEI Chao;LI Junhui;ZHENG Zhe;YANG Chuangye;DENG Yuewen(Fisheries College of Guangdong Ocean University,Zhanjiang 524088,Guangdong Province,China;Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province,Zhanjiang 524088,Guangdong Province,China;Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding,Zhanjiang 524088,Guangdong Province,China)
基金
supported by Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams,Department of Agriculture and Rural Affairs of Guangdong Province(Grant No.2020KJ146)
Special promotion of fishery science and technology of Ocean and Fisheries Bureau of Guangdong Province(Grant No.Z2014011 and Z2015002)
"Innovation Team Project"special funds(Grant No.2017KCXTD016)from the Department of Education of Guangdong Province
Modern Agricultural Industrial System(Grant No.CARS-049).
关键词
大珠母贝
类胡萝卜素
差异表达基因
Pinctada maxima
carotenoids
differential expression genes