高效液相色谱法测定粮食中真菌毒素的效果评价

Evaluation of the effect of high performance liquid chromatography on the determination of mycrotoxins in grain

本实验依据国家标准GB/T 5009.111,GB/T 5009.96和GB/T 5009.209中高效液相色谱法分别测定小麦样品中脱氧雪腐镰刀菌烯醇(DON)、赭曲霉毒素A(OTA)以及玉米赤霉烯酮(ZEN)这3种毒素的含量。按照检疫检验相关标准和文件要求,对该方法实践操作后结果的不确定性展开评定。结果表明:本次试验测定效果为DON﹥ZEN﹥OTA,其中OTA含量在该方法下无法准确衡量。最终通过计算不确定度,呕吐毒素和玉米赤霉烯酮的对应含量分别为(900±72.1)μg/kg和(60.12±3.85)μg/kg。

According to the national standard GB/T 5009.111,high performance liquid chromatography was used in GB/T 5009.96 and GB/T 5009.209 to determine the contents of deoxynienol(DON),chratoxinA(OTA)and zearalenone(ZEN)in wheat samples,respectively.According to the requirements of quarantine inspection standards and documents,the uncertainty of the results after the practice of this method was evaluated.The results showed that the determination effect was DON>ZEN>OTA,and OTA content could not be accurately measured by this method.By calculating,the corresponding contents of DON and ZEN were(900±72.1)μg/kg and(60.12±3.85)μg/kg,respectively.