自动粪便处理分析系统FA280临床应用分析

The Clinical Application Analysis of Automatic Fecal Analysis System FA280

目的评价自动粪便处理分析系统FA280在粪便常规检验中的应用价值。方法分析2020年4月20—25日本实验室在FA280上检测的粪便常规样本904例,及随后一年中58518例样本的寄生虫检出情况,通过人工识别审核和手工法复核,分析仪器与手工检测结果的一致性。结果FA280对粪便颜色、性状的识别与人工识别结果一致性较好(Kappa值为0.72、0.75)。FA280对红细胞、白细胞、真菌及脂肪滴的检出率均比人工识别仪器图像高,符合率均大于90%,除真菌外其余3项一致性较高(红细胞、白细胞和脂肪滴的Kappa值分别是0.814、0.718、0.986)。FA280对寄生虫的检出结果与手工结果符合率仅87.17%,一致性较差(Kappa值为0.112),但总体检出率为0.463%,大于文献报道的手工镜检寄生虫卵检出率0.311%。对于隐血检测,FA280报告结果与人工读取结果符合率高达99.78%,一致性非常好(Kappa值为0.994)。结论FA280对粪便常规样本的检测与手工检测结果大部分一致,且提高了检测效率,在样本筛查中具有较好的临床应用价值。

Objective To evaluate the application value of automatic fecal analysis system FA280 in routine fecal examination.Methods A total of 904 routine fecal specimens detected on FA280 in our laboratory from April 20 to 25,2020 and the detection of parasites in 58518 specimens in the following year was analyzed.The consistency of the instrument and manual test results was analyzed through manual identification and manual review.Results FA280 had a good consistency with artificial identification of fecal color and traits(Kappa value was 0.72 and 0.75).The detection rate of FA280 on red blood cells,white blood cells,fungi and fat droplets was higher than that of artificial identification,the coincidence rate was more than 90%,except for fungi,the other three had high consistency(Kappa values of red blood cells,white blood cells and fat droplets were 0.814,0.718 and 0.986,respectively).The coincidence rate of parasite report between FA280 and manual method was only 87.17%,with poor consistency(Kappa value was 0.112),but the overall detection rate of parasite was 0.463%,which was higher than the manual microscopic detection rate of 0.311%reported in the literature.When reading the results of occulting blood reagent card,the coincidence rate of FA280 and manual method was up to 99.78%,showing a good consistency(Kappa value was 0.994).Conclusion The detection results of fecal specimens by FA280 is mostly consistent with the results of manual method,and the detection efficiency is improved,so that it had great clinical application value for sample screening.