杨梅黄酮对变形链球菌毒力因子及生物膜的抑制作用研究

Effect of Myricetin on Virulence Factor and Biofilm of Streptococcus Mutans

目的:探讨杨梅黄酮对变形链球菌(Streptococcus mutans,S.mutans)ATCC 25175毒力因子和生物膜的作用。方法:用液体稀释法准确测定杨梅黄酮对变形链球菌的最小抑菌浓度(MIC)和耐酸性;用结晶紫染色法检测药物对变形链球菌生物膜形成的抑制率;用乳酸试剂盒检测药物对变形链球菌产酸的影响;用蒽酮法检测药物对不溶性多糖生成量的影响;利用场发射扫描电子显微镜(FESEM)和激光共聚焦显微镜(CLSM)观察及分析药物对生物膜的抑制作用。结果:杨梅黄酮对变形链球菌的最小抑菌浓度为0.8 mg/mL,0.2~0.4 mg/mL的药物对生物膜的生成抑制作用明显,同时乳酸、不溶性多糖的生成量明显降低,0.4 mg/mL的药物对细菌耐酸性以及成熟生物膜的影响有显著性差异。结论:杨梅黄酮对于变形链球菌毒力因子和生物膜有显著的抑制作用。

Objective:To study the inhibitory effect of myricetin on virulence factors and biofilm of Streptococcus mutans(S.mutans).Methods:S.mutans ATCC 25175 was used in this study.Dilution method was used to test MIC and acid tolerance.The inhibition rate on biofilm was measured by crystalline violet staining.The acid production was measured by lactic acid kit.Anthrone method was conducted to determine the production of EPS.FESEM and CLSM were used to observed the formation of biofilm after treated by myricetin.Results:The MIC of myricetin on S.mutans was 0.8 mg/mL,and the inhibitory effect of myricetin from 0.2 mg/mL to 0.4 mg/mL on the production of biofilm was obvious,while the production of lactic acid and insoluble polysaccharide significantly reduced,and the effect of myricetin at 0.4 mg/mL on bacterial acid tolerance was significantly different.Conclusion:Myricetin plays a significant part on virulence factor and biofilm of S.mutans.