人舌鳞状细胞癌细胞系WU-TSC-1的建立及鉴定

Establishment and Identification of Human Tongue Squamous Cell Carcinoma Cell Line WU-TSC-1

目的:建立1株人舌鳞状细胞癌细胞系WU-TSC-1并鉴定其基本生物学特性。方法:将所获得的舌鳞状细胞癌患者手术切除的组织块标本进行原代培养,传代后建立舌鳞状细胞癌细胞系WU-TSC-1,显微镜下观察并记录其细胞形态和生长特性,行基因组DNA短串联重复序列分析,并对其倍增时间、凝集反应、细胞表面标记物、染色体核型和裸鼠成瘤能力等特性进行检测。结果:WU-TSC-1可稳定传代50余代,为人源肿瘤细胞,无其他细胞交叉污染。细胞呈卵圆形或方圆形,失去接触抑制。染色体核型为非二倍体染色体。体外细胞倍增时间为51.15 h,体内具有成瘤能力。结论:该研究成功建立了1株人舌鳞状细胞癌细胞系WU-TSC-1,为揭示舌鳞状细胞癌的发病机制,探索诊断指标和治疗方案提供了新的实验细胞系。

Objective:To establish a human tongue squamous cell carcinoma(TSCC)cell line WU-TSC-1 and to identify its basic biological characteristics.Methods:The excised tissue from a patient with TSCC was primary cultured.After passage,the TSCC cell line WU-TSC-1 was established.Its morphology and growth characteristics were observed and recorded through microscope.Short tandem repeat analysis(STR)of genomic DNA on this cell was conducted.Its doubling time,agglutination reaction,cell surface markers,chromosome karyotype,and tumorigenic ability in nude mice were also examined.Results:WU-TSC-1 cells had been subcultured for more than 50 passages which were confirmed to be human-derived tumor cells with no other cell contamination.In vitro,the cells lost contact inhibition and had a uniform oval or squares shape and non-diploid chromosomes.Its doubling time was calculated as 51.15 h.In vivo,the cell line showed tumorigenesis ability.Conclusion:This study has successfully established a human TSCC cell line WU-TSC-1 which provided a new cell line for revealing the pathogenesis of TSCC as well as exploring useful diagnostic indicators and treatment options.