基于Nrf2通路研究二仙解毒方对弱精子症大鼠精子微环境的影响

Study on the Effect of Erxian Jiedu Recipe on the Sperm Microenvironment of Rats with Asthenospermia Based on Nrf2 Pathway

目的基于Nrf2信号通路研究二仙解毒方对弱精子症模型大鼠精子微环境的改善作用及可能机制。方法使用奥硝唑法构建成熟雄性大鼠弱精子症模型,随机分为模型组、二仙解毒方低剂量组、中剂量组、高剂量组、左卡尼汀组,每组各12只,连续灌胃4周后,检测各组大鼠精子浓度、精子活动力、相关氧化应激指标、大鼠睾丸及附睾中基于Nrf2蛋白表达及mRNA表达。结果二仙解毒方可增强弱精子症模型大鼠精子活动力,剂量越高,氧化应激指标改变越显著,二仙解毒方高剂量组可将模型组大鼠的精液抗氧化能力恢复至正常大鼠水平;二仙解毒方可促进大鼠附睾、睾丸组织Nrf2基因转录与蛋白表达。结论二仙解毒方确有提高精子活动力的作用,其可能的作用机制为通过诱导上调生殖细胞内Nrf2 mRNA和蛋白表达,以改善弱精子症大鼠精液氧化应激状态,从而提高精子活动力。

Objective Based on the Nrf2 signal pathway to study the effect of Erxian Jiedu Recipe on the sperm microenvironment of rats with asthenospermia and its possible mechanism.Methods The ornidazole method was used to construct a mature male rat model of asthenospermia,which was randomly divided into model group,Erxian Jiedufang low-dose group,Erxianjiedufang medium-dose group,Erxianjiedufang high-dose group,and L-carnitine In each group,12 rats in each group.After continuous gastric administration for 4 weeks,the sperm concentration,sperm motility,related oxidative stress indicators,Nrf2 protein expression and mRNA expression in the rat testis and epididymis were detected in each group.Results Erxian Jiedu Decoction can enhance sperm motility in rats with asthenospermia.The higher the dose,the more significant the changes in oxidative stress indicators.The high-dose Erxian Jiedu Decoction can restore the semen antioxidant capacity of the model group to Normal rat level;Erxian Jiedu Decoction can promote Nrf2 gene transcription and protein expression in rat epididymis and testis tissues.Conclusion Erxian Jiedu Recipe does improve sperm motility.Its possible mechanism is to improve the oxidative stress state of semen in asthenospermia rats by inducing up-regulation of Nrf2 mRNA and protein expression in germ cells,thereby improving sperm motility.