益肾化浊方对快速老化P8小鼠学习记忆能力及精神行为影响

Effects of Yishen Huazhuo Decoction on Learning and Memory Ability and Psychiatric Behavior in Senescence-Accelerated Mouse-Prone 8

目的观察中药益肾化浊方对快速老化P8(SAMP8)小鼠学习记忆能力及精神行为的影响。方法将20只3月龄雄性SAMP8小鼠随机分为模型(SAMP8)组和益肾化浊方(YSHZ)组,每组10只,并选用10只抗快速老化小鼠(SAMR1)作为对照。YSHZ组予益肾化浊方按生药6.24g/(kg·d)进行灌胃,其他两组予以等量蒸馏水,每天1次,持续12周。采用Morris水迷宫评价小鼠的学习记忆能力,旷场实验和高架十字迷宫评价其精神行为情况。结果水迷宫实验显示,各组小鼠平均游泳速度无统计学差异(P>0.05);与SAMR1组相比,SAMP8组小鼠逃避潜伏期显著延长、穿越平台次数显著减少(P<0.05);与SAMP8组相比,YSHZ组小鼠避潜伏期显著缩短、穿越平台次数显著增加(P<0.05)。旷场实验和高架十字迷宫结果显示,与SAMR1组相比,SAMP8组小鼠总运动距离、进入中心区域次数及在开放臂停留的时间比例均显著减少(P<0.05);与SAMP8组相比,YSHZ组小鼠总运动距离和进入中心区域次数均显著增加(P<0.05),在开放臂停留的时间比例有增加的趋势(P>0.05)。结论益肾化浊方可有效改善SAMP8小鼠早期学习记忆能力减退及精神行为异常。

Objective To observe the effect of Yishen Huazhuo Decoction on the learning and memory ability and psychiatric behavior of senescence-accelerated-prone mouse-8(SAMP8).Methods Twenty 3-month-old male SAMP8 mice were randomly divided into model group(SAMP8)and Yishen Huazhuo Decoction(YSHZ)group,with 10 mice in each group.Ten 3-month-old male senescence-accelerated mouse resistant 1(SAMR1)mice were used as the control group(SAMR1).YSHZ group was given Yishen Huazhuo Decoction by 6.24 g/(kg·d)of crude drug,while SAMP8 and SAMR1 groups were given the same amount of distilled water once a day for 12 weeks.Morris water maze(MWM)was applied to evaluate the learning and memory ability of the mice.Open field test(OFT)and elevated plus maze(EPM)were employed to measure their psychiatric behavior.Results The results of MWM showed that there was no significant difference in the average swimming speed among the groups(P>0.05).Compared with SAMR1 group,the escape latency of SAMP8 group was significantly prolonged and the number of crossing platform was significantly reduced(P<0.05).Compared with SAMP8 group,the escape latency of YSHZ group was significantly shortened and the number of crossing platform was significantly increased(P<0.05).The results of OFT and EPM revealed that compared with the SAMR1 group,the total movement distance,the number of times to enter the central zone and the proportion of time to stay in the open arms of SAMP8 group were significantly reduced(P<0.05),while compared with SAMP8 group,the first two indicators of YSHZ group were significantly increased(P<0.05),and the proportion was increased(P>0.05).Conclusion Yishen Huazhuo Decoction can effectively improve the early learning and memory impairment and psychiatric disorders of SAMP8 mice.