基于网络药理学探讨益气凉血生肌方预防冠心病PCI术后复发心绞痛机制

Network Pharmacology of Yiqi Liangxue Shengji Decoction Protecting against Recurrence of Angina after Angiographically Percutaneous Coronary Intervention

目的基于网络药理学研究益气凉血生肌方预防冠心病PCI术后复发心绞痛药效机制。方法通过TCMSP,获取该方各药有效成分及各个成分对应的靶点,并对成分-靶点相互作用结果进行可视化,“心绞痛”相关靶点通过Genecards和OMIM获取;通过VENN图取药物与疾病靶点交集,将交集靶点提交至String获取PPI图以及相关文件,进一步通过CytoNCA插件获得核心靶点。运用Metascape对核心靶点进行GO和KEGG分析,最后对其结果进行可视化。结果挖掘到药物和疾病相关靶点分别为252个和1740个,交集靶点166个、核心靶点26个。其中槲皮素、山奈酚与木犀草素等异黄酮成分排名靠前,可能通过RELA、MAPK14、MAPK1等靶点作用于TNF、IL-17以及糖尿病并发症AGE-RAGE等信号通路发挥作用。结论揭示了益气凉血生肌方可能通过多成分、多靶点、多通路作用机制预防PCI术后复发心绞痛。

Objective To explore the biological mechanism of Yiqi Liangxue Shengji Decoction protecting against recurrence of angina after angiographically percutaneous coronary intervention by network pharmacology.Methods The effective components and corresponding targets of Yiqi Liangxue Shengji Decoction were screened by TCMSP database and Component-target network was constructed by Cytoscape software.Then searching angina related targets from OMIM and GeneCards database.protein-protein interaction network was analyzed using STRING database,and the core target is obtained through the CytoNCA plug-in.Results After selection,252 potential target genes of Yiqi Liangxue Shengji Decoction and 1740 for AP were obtained.The active ingredients,such as kaempferol,quercetin,and luteolin mainly act on RELA,MAPK14,MAPK1 and other key targets.Through fluid shear stress atherosclerosis,TNF,IL-17 signaling pathway and other signaling pathways,playing anti-inflammatory and other functions in the treatment of angina pectoris.Conclusion The potential mechanism of Yiqi Liangxue Shengji Decoction for recurrence of angina after angiographically percutaneous coronary intervention has been explored based on the multi-component,which provides a basis for the follow-up scientific research and clinical application of Yiqi Liangxue Shengji Decoction in the treatment of angina and extended indications.