虎杖3种主要化合物单体对大鼠痛风性关节炎的预防及治疗作用

Preventive and therapeutic effects of 3 compound monomers of Polygonum cuspidatum on gouty arthritis in rats

目的研究虎杖提取物中虎杖苷、白藜芦醇、大黄素3种主要化合物单体对痛风性关节炎模型大鼠的抗炎作用。方法72只SD大鼠按体重随机分为12组(空白对照组,模型组,阳性对照组,虎杖苷低、中、高剂量组,白藜芦醇低、中、高剂量组及大黄素低、中、高剂量组),每组6只。空白对照组、模型组大鼠予生理盐水,阳性对照组予吲哚美辛10 mL/kg,3种化合物单体低、中、高剂量组分别予对应药物125、250、500 mg/kg,各组大鼠均连续灌胃给药14 d。除空白对照组外,其余各组均于给药第12天时在大鼠右后足趾关节注射尿酸钠溶液建立痛风性关节炎大鼠模型。建模后第3天对大鼠右后足趾关节组织进行组织病理学检查,以评价关节的炎症反应和结晶体病变情况。采用ELISA检测大鼠血清中TNF-α、IL-6和IL-10的水平。结果造模后4~48 h各组大鼠的关节肿胀程度均高于空白对照组(P均<0.05),除空白对照组外,其余各组大鼠的关节肿胀程度均随时间推移先升高后降低。组织病理结果显示,虎杖苷低剂量组及白藜芦醇、大黄素3个剂量组大鼠关节部位仍可见以炎症反应为主的病理变化,但与模型组相比,上述各组发生炎症反应和结晶体沉积的大鼠只数减少、炎症反应和结晶体沉积的严重程度降低。此外,与模型组比较,虎杖中的虎杖苷、白藜芦醇和大黄素3种化合物单体对痛风性关节炎大鼠血清中TNF-α、IL-6、IL-10的影响不同,但均具有一定的抗炎效果。结论虎杖中的虎杖苷、白藜芦醇和大黄素3种单体均对痛风性关节炎模型大鼠具有预防及治疗炎症的作用。

Objective To study the anti-inflammatory effects of polydatin,resveratrol and emodin in Polygonum cuspidatum(P.cuspidatum)extract on gouty arthritis in rats.Methods A total of 72 SD rats were randomly divided into 12 groups(blank control group,model group,positive control group,low-,medium-and high-dose groups of polydatin,low-,medium-and high-dose groups of resveratrol,and low-,medium-and high-dose groups of emodin),with 6 rats in each group.Rats in the blank control group and model group were given normal saline,the positive control group was given indomethacin 10 mL/kg,and the low-,medium-and high-dose groups of the 3 compound monomers were intragastrically given corresponding drugs 125,250 and 500 mg/kg,for 14 consecutive days.The gouty arthritis rats were established by injecting sodium urate solution into the right posterior toe joint on the 12^(th) day of administration.On the 3^(rd) day after modeling,histopathological examination was performed on the rat’s toe joint tissue to evaluate the inflammation reaction and crystalline lesions of the joint.Serum tumor necrosis factor(TNF)-α,interleukin(IL)-6 and IL-10 levels were detected by enzymelinked immunosorbent assay.Results The degrees of joint swelling of rats in each model group were higher than that of the blank control group(all P<0.05)at 4-48 h after modeling.Except for the blank control group,the degrees of joint swelling of rats in other groups increased first and then decreased over time.Histopathological results showed that pathological changes dominated by inflammatory reaction were still observed in the joints of rats in the low-dose group of polydatin and 3 dose groups of resveratrol and emodin,but the incidence and severity of inflammatory reaction and crystal lesions were significantly decreased in each treatment group compared with the model group.In addition,compared with the model group,polydatin,resveratrol and emodin in P.cuspidatum had different effects on the serum TNF-α,IL-6 and IL-10 in gouty arthritis rats and they all had obvious anti-inflammatory effects.Conclusion Polydatin,resveratrol and emodin in P.cuspidatum all have effects of preventing and treating inflammation in rats with gouty arthritis.