海带褐藻多酚对绿烂海带表面分离的一株细菌的抑菌效果研究

THE ANTIBACTERIAL ACTIVITY OF PHLOROTANNINS AGAINST A BACTERIA ISOLATE FROM BROWN ALGA SACCHARINA JAPONICA SUFFERED WITH GREEN-ROTTEN DISEASE

海带苗绿烂病暴发严重威胁海带养殖生产,给海带养殖业造成巨大经济损失。研究发现,海带中的多酚化合物可以抵御海带病害初期病原体侵入。为研究多酚对绿烂海带表面附生菌的抑菌效果,在病烂部位分离出一株细菌,通过16s rRNA基因测序的方法,证实该细菌为小孔芽孢杆菌(Bacillus foraminis)。进一步以健康海带为原料提取多酚,利用平板生长抑制法,以哈维氏弧菌(Vibrio harveyi)和溶藻弧菌(Vibrioalginolyticus)作为对照,分析多酚对小孔芽胞杆菌的抑菌效果。结果显示褐藻多酚在接种了哈维氏弧菌和溶藻弧菌的培养基上产生了不同大小的抑菌圈,而在接种有小孔芽孢杆菌的培养基上未产生抑菌圈,表明多酚对小孔芽胞杆菌无抑菌效应。此项研究为海带多酚抑菌作用提供了参考,同时对了解海带病害暴发及开展海带病害防治工作具有重要意义。

The outbreak of green-rotten kelp seedlings seriously threatens kelp breeding production and causes huge economic losses to kelp breeding industry.It is found that phlorotannins in kelp can resist the invasion of pathogens in the early stage of kelp disease.To study the antibacterial effect of phlorotannins on epiphytes on the surface of green-rotten kelp,a strain of bacteria was isolated from the rotten part.It was confirmed that the bacterium was Bacillus foraminis by 16S rRNA gene sequencing.Furthermore,polyphenols were extracted from healthy kelp,and the inhibitory effect of phlorotannins on B.foraminis was analyzed by plate growth inhibition assay,with Vibrio harveyi and Vibrio alginolyticus as controls.Results show that phlorotannins produced different sizes of inhibitory circles on the medium inoculated with V.harveyi and V.alginolyticus,but show no inhibitory effect on B.subtilis.This study provides a reference for the bacteriostatic effect of phlorotannins,and is of great significance to understand the outbreak of kelp diseases and carry out the prevention and control of kelp diseases.