结直肠癌患者Kras基因突变与分化程度及组织类型的相关性研究

Correlation between Kras gene mutation,differentiation degree and tissue type in patients with colorectal cancer

目的:探究结直肠癌KRAS基因突变与分化程度及组织类型的相关性研究。方法:选取结直肠癌患者89例,其中26例KRAS突变阳性患者纳入突变组,63例KRAS突变阴性且无其他突变患者纳入未突变组。采用荧光定量PCR Taqman-ARMS探针法测定KRAS基因突变,比较组间的差异性与相关性。结果:结直肠癌患者KRAS突变阳性率的分析结果显示,结直肠癌KRAS阳性率为29.21%;KRAS基因突变在普通腺癌的检出率高于黏液性腺癌,高分化、中分化和低分化的程度与突变与否有统计学差异(P<0.05),在突变组中中分化的比例较高。结论:结直肠癌患者KRAS突变组织学类型和分化程度有明显相关性。

Objective:To explore the correlation between Kras gene mutation,differentiation degree and tissue type in patients with colorectal cancer.Methods:89 patients diagnosed with colorectal cancer were retrospectively studied,of shom,26 patients with Kras mutation positive were included in the mutation group,and 63 patients with negative Kras mutations and with no other mutations were included in the non-mutant group.Kras gene mutations were detected by fluorescence quantitative PCR TaqMan-ARMS probe,and their differences and correlations were compared between groups.Results:The positive rate of Kras mutation in patients with colorectal cancer was analyzed,and the results showed that 29.21%patients were with Kras mutation positive;the detection rate of Kras gene mutation in common adenocarcinoma was higher than that in mucinous adenocarcinoma,and there was statistical difference between the degree of high,medium and low differentiation,and whether they were mutated or not(P<0.05),the proportion of differentiation was higher in the medium mutant group.Conclusion:There is a significant correlation between the histological type and degree of differentiation of Kras mutations in colorectal cancer patients.