miR-155 HG在寻常型银屑病患者外周血单个核细胞中的表达水平及其与Th17/treg细胞平衡的关系

Expression of miR-155 HG in Peripheral Blood Mononuclear Cells of Patients with Psoriasis Vulgaris and its Relationship with Th17/treg Cell Balance

【目的】探讨microRNA-155宿主基因(miR-155 HG)在寻常型银屑病患者外周血单个核细胞(PBMC)中的表达水平及其与辅助性T细胞17/调节性T淋巴细胞(Th17/treg)细胞平衡的关系。【方法】选择2020年10月至2021年10月在榆林市第二医院诊治的寻常型银屑病患者92例血液标本为疾病组,另外选取同期健康体检者92例血液标本为对照组,采用实时定量PCR(RT-PCR)检测PBMC中miR-155 HG、miR-155表达水平,采用流式细胞仪检测Th17、treg细胞比例,计算Th17/treg值;采用酶联免疫吸附试验(ELISA)检测血清白细胞介素-6(IL-6)、转化生长因子β1(TGF-β1)水平;采用Pearson法分析PBMC中miR-155 HG、miR-155水平及其与Th17/treg值、IL-6、TGF-β1水平的相关性。【结果】与对照组比较,疾病组患者PMBC中miR-155 HG、miR-155、IL-6水平及Th17/treg值均显著增加(P<0.05),血清TGF-β1水平降低(P<0.05)。疾病组患者PBMC中miR-155 HG水平与miR-155水平呈正相关(r=0.573,P=0.000);miR-155 HG水平与Th17/treg值、IL-6水平呈正相关(r=0.482、0.512,P=0.000),与TGF-β1水平呈负相关(r=-0.596,P=0.000);miR-155水平与Th17/treg值、IL-6水平呈正相关(r=0.694、0.488,P=0.000),与TGF-β1水平呈负相关(r=-0.509,P=0.000)。【结论】寻常型银屑病患者PBMC中miR-155 HG水平上调,可能与分泌miR-155、Th17/treg细胞失衡有关。

【Objective】To investigate the expression level of microRNA-155 host gene(miR-155 HG)in peripheral blood mononuclear cells(PBMC)of patients with psoriasis vulgaris and its relationship with the balance of helper T cell 17/regulatory T lymphocyte(Th17/treg)cells.【Methods】A total of 92 blood samples from patients with psoriasis vulgaris treated in the Yulin Second Hospital from October 2020 to October 2021 was selected as disease group,and 92 blood samples from healthy people in the same period were selected as control group.The expression levels of miR-155,Hg and miR-155 in PBMC were detected by real-time quantitative PCR(RT-PCR),and the proportion of Th17 and treg cells was detected by flow cytometry,Th17/treg valu was calculated;Serum interleukin-6(IL-6)and transforming growth factorβ1(TGF-1)were detected by enzyme-linked immunosorbent assay(ELISA);The levels of miR-155 and miR-155 HG in PBMC and their relationship with Th17/treg value,IL-6 and TGF-β1 were analyzed by Pearson method.【Results】Compared with the control group,the levels of miR-155,miR-155 HG,IL-6 and Th17/treg in PMBC in the disease group were significantly increased(P<0.05),and serum TGF-β1 decreased(P<0.05).Of patients in the disease group,the level of miR-155 HG in PBMC was positively correlated with that of miR-155(r=0.573,P=0.000);The level of miR-155 HG was positively correlated with Th17/treg value and IL-6 level(r=0.482,0.512,P=0.000),and was negatively correlated TGF-β1(r=-0.596,P=0.000);The level of miR-155 was positively correlated with Th17/treg value and IL-6 level(r=0.694,0.488,P=0.000),and was negatively correlated with TGF-β1 level(r=-0.509,P=0.000).【Conclusion】The level of miR-155 HG in PBMC of patients with psoriasis vulgaris is up-regulated,which may be related to the imbalance of secreting miR-155 and Th17/treg cells.