姜黄素对糖尿病小鼠认知功能障碍的影响

Effects of Curcumin on cognitive dysfunction in diabetic mice

目的研究姜黄素(Curcumin)对糖尿病小鼠认知功能障碍的改善作用。方法选8 w雄性C57BL/6J小鼠40只,随机分成正常对照组10只、模型组15只、Curcumin治疗组15只[200 mg/(kg·d),灌胃]。采取高脂联合链脲佐菌素(Streptozotocin,STZ)(60 mg/kg)的方法建立糖尿病小鼠认知障碍模型。2 w后测定小鼠体质量、空腹血糖(fasting plasma glucose,FBG)及葡萄糖耐量;采用Morris水迷宫、Y迷宫、新颖物体识别实验测试小鼠的认知能力。处死小鼠后,测定小鼠海马组织中丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)活性和肿瘤坏死因子α(tumor necrosis factorα,TNF-α)的水平,HE染色观察脑组织病理形态学变化。结果模型组小鼠较对照组体质量降低(t=25.71,P<0.01)、血糖升高(t=32.76,P<0.01),葡萄糖耐量明显异常(t=305.67,P<0.01),逃避潜伏期时间(escape latent period,ELP)延长(t=12.76,P<0.05),正确交替率及新颖物体识别指数明显下降(t=36.98、42.17,P<0.01),海马组织中MDA活性增加,TNF-α水平升高,SOD活性降低(t=17.38、21.83、14.75,均P<0.05)。Curcumin组小鼠ELP显著缩短(t=28.97,P<0.05),而在原平台象限停留的时间明显延长(t=5.84,P<0.05),正确交替率、新颖物体识别指数显著上升(t=32.89、45.27,P<0.01);海马组织中MDA活性降低,SOD活性升高,TNF-α水平下降(t=23.11、12.75、14.36,均P<0.05)。结论姜黄素通过减轻炎症反应和提高抗氧化能力发挥改善糖尿病小鼠认知功能障碍的作用。

Objective To study the improvement effect of Curcumin on cognitive dysfunction in diabetic mice.Methods Forty male C57 BL/6 J mice aged 8 w were randomly divided into normal control group with 10 cases,model group with 15 cases and Curcumin treatment group with 15 cases[200 mg/(kg·d)].Diabetic mice cognitive dysfunction model was established by hyperlipidemia combined with Streptozotocin(STZ)(60 mg/kg).The body weight,fasting plasma glucose(FBG)and fasting glucose tolerance of mice after 2 w were measured.Morris water maze,Y maze and novel object recognition experiments were used to test the cognitive ability of mice.The levels of malondialdehyde(MDA),superoxide dismutase(SOD)and tumor necrosis factorα(TNF-α)were determined in the hippocampal tissues of mice after death,and HE staining was used to observe pathological change in cerebral tissues.Results Compared to the control group,the body weight of mice in the model group decreased(t=25.71,P<0.01),blood glucose increased(t=32.76,P<0.01),glucose tolerance was evidently abnormal(t=305.67,P<0.01),the escape latent period(ELP)was prolonged(t=12.76,P<0.05),the correct alternation rate and novel object recognition index significantly decreased(t=36.98,42.17,P<0.01).The increase of MDA activity and TNF-αlevel and decrease of SOD activity were found in hippocampus(t=17.38,21.83,14.75,P<0.05).ELP was significantly shortened in Curcumin group(t=28.97,P<0.05),while the residence time in the original platform quadrant was significantly prolonged(t=5.84,P<0.05),and the correct alternation rate and novel object recognition index were significantly increased(t=32.89,45.27,P<0.01).MDA activity decreased,SOD activity increased and TNF-αlevel decreased in hippocampus(t=23.11,12.75,14.36,P<0.05).Conclusion Curcumin is effective in improving cognitive dysfunction in diabetic mice by reducing inflammatory response and improving antioxidant capacity.