辣椒愈伤组织的诱导及再生体系的建立

Establishment of Callus Induction and Regeneration System of Capsicum

目的:筛选高效诱导辣椒‘凤紫运1号’愈伤组织及再生体系的培养基,为遗传转化体系的建立奠定基础。方法:以‘凤紫运1号’辣椒叶片为材料,在培养基(MS)中添加不同试剂筛选出诱导愈伤组织的最佳体系,添加不同浓度激素筛选出愈伤组织分化最佳激素组合及再生体系的建立。结果:MS+(25 g/L蔗糖)+(0.3 mg/L 6-BA)+(0.4 mg/L IBA)组合可诱导出淡绿色致密颗粒状愈伤组织,且诱导率为83%;MS+(30 g/L蔗糖)+(0.1 mg/L 6-BA)+(0.1 mg/L NAA)组合诱导辣椒愈伤组织分化率为58.33%;MS+(30 g/L蔗糖)+(0.3 mg/L IAA)组合诱导不定芽增殖率为56.32%,平均增殖数为2.5个;MS+(30 g/L蔗糖)+(0.05 mg/L NAA)诱导不定芽生根率为93.73%,平均根长为2.745 cm。结论:‘凤紫运1号’辣椒愈伤组织诱导的最佳培养基为MS+(25 g/L蔗糖)+(0.3 mg/L 6-BA)+(0.4 mg/L IBA)组合;愈伤组织分化的最佳培养基为MS+(30 g/L蔗糖)+(0.1 mg/L 6-BA)+(0.1 mg/L NAA)组合;诱导不定芽增殖的最佳培养基为MS+(30 g/L蔗糖)+(0.3 mg/L IAA)组合;诱导生根的最佳培养基为MS+(30 g/L蔗糖)+(0.05 mg/L NAA)组合。

Objective: To screen the medium for inducing callus and regeneration system of Pepper ‘fengziyun No.1’, and lay a foundation for the establishment of genetic transformation system. Methods: Using the leaves of ‘fengziyun No.1’ pepper as materials, different reagents were added to the culture medium to screen the best system for callus induction, and different concentrations of hormones were added to screen the best hormone combination for callus differentiation and the establishment of regeneration system. Results: Light green dense granular callus was induced by MS+(25 g/L sucrose)+(0.3 mg/L 6-BA)+(0.4 mg/L IBA) combination, and the induction rate was 83%. The callus differentiation rate of pepper induced by MS+(30 g/L sucrose)+(0.1 mg/L 6-BA)+(0.1 mg/L NAA) was combinoction 58.33%. The proliferation rate of adventitious buds induced by MS+(30 g/L sucrose)+(0.3 mg/L IAA) combinoction was 56.32%, and the average proliferation number was 2.5. The rooting rate of adventitious buds induced by MS+(30 g/L sucrose)+(0.05 mg/L NAA) combinoction was 93.73%, and the average root length was 2.745 cm. Conclusion: The best medium for callus induction of ‘fengziyun 1’ pepper was MS +(25 g/L sucrose)+(0.3 mg/L 6-BA)+(0.4 mg/L IBA) combinoction. The best medium for callus differentiation was MS+(30 g/L sucrose)+(0.1 mg/L 6-BA)+(0.1 mg/L NAA) combinoction. The best medium for inducing adventitious bud proliferation was MS+(30 g/L sucrose)+(0.3 mg/L IAA) combinoction. The best medium for inducing rooting was MS+(30 g/L sucrose)+(0.05 mg/L NAA).