息风定喘方对哮喘豚鼠血清VEGF、EGF、TGF-β2的调节作用

Regulatory effect of Xifeng Dingchuan Prescription on VEGF,EGF and TGF-β2 in serum of asthmatic guinea pigs

目的研究息风定喘方对哮喘豚鼠血管内皮生长因子(VEGF)、表皮生长因子(EGF)、转化生长因子β2(TGF-β2)的调节作用,探讨其作用机制。方法将72只普通级雄性豚鼠按照随机数字表法分为正常对照组、寒哮模型组、息风定喘方高剂量组[22.72 g/(kg·d)]、息风定喘方中剂量组[11.36 g/(kg·d)]、息风定喘方低剂量组[5.68 g/(kg·d)],地塞米松组[0.5 mg/(kg·d)],每组12只。除正常对照组外利用卵清蛋白致敏+寒冷刺激复制寒性哮喘豚鼠模型,正常对照组给予等剂量生理盐水,造模成功后于实验第29天起,息风定喘方高、中、低剂量组灌胃给药,正常对照组和寒哮模型组予生理盐水。观察各组豚鼠的一般情况,HE染色法检测息风定喘方对寒性哮喘豚鼠模型的治疗效果;酶联免疫吸附试验分析血清VEGF、EGF、TGF-β2的含量。结果息风定喘方高剂量组豚鼠症状改善情况优于息风定喘方中、低剂量组。与寒哮模型组比较,息风定喘方各剂量组气道改变均有减轻,有不同程度炎症细胞浸润,肺泡间隔厚度降低。与正常对照组比较,寒哮模型组血清中VEGF、EGF、TGF-β2含量显著升高,差异均有高度统计学意义(P<0.01)。与寒哮模型组比较,息风定喘方各剂量组的VEGF、EGF、TGF-β2含量均降低,差异有统计学意义(P<0.05)。息风定喘方低、中剂量组血清VEGF含量高于息风定喘方高剂量组,差异均有统计学意义(P<0.05)。息风定喘方低剂量组血清EGF含量高于息风定喘方高、中剂量组,差异有统计学意义(P<0.05)。息风定喘方低剂量组血清TGF-β2含量高于息风定喘方高、中剂量组,息风定喘方中剂量组血清TGF-β2含量高于息风定喘方高剂量组,差异有统计学意义(P<0.05)。结论息风定喘方可通过调节VEGF、EGF、TGF-β2含量,达到降低炎症因子水平,控制气道炎症,干预气道重塑的目的。

Objective To study the regulatory effect of Xifeng Dingchuan Prescription on vascular endothelial growth factor(VEGF),epidermal growth factor(EGF)and transforming growth factor β2(TGF-β2)in asthmatic guinea pigs and explore its mechanism of action.Methods Seventy-two general grade male guinea pigs were divided into normal control group,cold asthma model group,high dose group[22.72 g/(kg·d)],medium dose group[11.36 g/(kg·d)],low dose group[5.68 g/(kg·d)]of Xifeng Dingchuan Prescription,Dexamethasone group[0.5 mg/(kg·d)]according to random number table method,with 12 guinea pigs in each group.In addition to the normal control group,ovalbumin sensitization+cold stimulation was used to replicate the guinea pig model of cold asthma.The normal control group was given equal dose of normal saline.On the 29th day of the experiment,the high,medium and low dose groups of Xifeng Dingchuan Prescription were given orally,while normal control group and cold asthma model group were given normal saline.The general situation of guinea pigs in each group was observed.HE staining was used to detect the therapeutic effect of Xifeng Dingchuan prescription on guinea pig model of cold asthma.The contents of VEGF,EGF,and TGF-β2 in serum were analyzed by enzyme linked immunosorbent assay.Results The improvement of guinea pig symptoms in high dose group of Xifeng Dingchuan Prescription was better than medium and low dose group of Xifeng Dingchuan Prescription.Compared with the cold asthma model group,airway changes in all doses of Xifeng Dingchuan Prescription groups were alleviated,inflammatory cell infiltration was observed in different degrees,and alveolar septum thickness was decreased.Compared with normal control group,the levels of VEGF,EGF,and TGF-β2 in serum of cold asthma model group were significantly increased,with high statistical significance(P<0.01).Compared with the cold asthma model group,the contents of VEGF,EGF,and TGF-β2 in all doses of Xifeng Dingchuan Prescription groups were decreased,with statistical significance(P<0.05).The serum VEGF content in the low dose and medium dose groups of Xifeng Dingchuan Prescription was higher than that in the high dose group of Xifeng Dingchuan Prescription,with statistical significance(P<0.05).The serum EGF content in the low dose group was higher than that in the high dose and medium dose groups of Xifeng Dingchuan Prescription,with statistical significance(P<0.05).The content of TGF-β2 in serum of low dose group of Xifeng Dingchuan Prescription was higher than that of high dose and medium dose groups of Xifeng Dingchuan Prescription,and the content of TGF-β2 in serum of medium dose group of Xifeng Dingchuan Prescription was higher than that of high dose group of Xifeng Dingchuan Prescription,with statistical significance(P<0.05).Conclusion By regulating the content of VEGF,EGF,and TGF-β2,Xifeng Dingchuan can reduce the level of inflammatory factors,control airway inflammation and intervene airway remodeling.