溃疡性结肠炎中巨噬细胞差异基因及功能分析

Analysis of differential genes and functions of macrophages in ulcerative colitis

目的研究溃疡性结肠炎(UC)肠道巨噬细胞的基因表达差异和生物学功能。方法选取2019年1月至12月前往the VA San Diego医疗机构就诊的7例UC患者和7名健康对照者为研究对象。分析直肠黏膜单细胞测序数据,比较巨噬细胞的差异表达基因,进行基因本体(GO)功能富集和京都基因和基因组数据库(KEGG)信号通路富集分析。结果过滤细胞,确定巨噬细胞群。筛选出UC组和对照组肠黏膜差异基因31个,包括TIMP1、IFITM3、IGHG1等。对差异基因进行GO和KEGG富集分析,发现主要富集于氧化应激生物学过程和IL-17信号通路。结论肠道巨噬细胞的氧化应激和IL-17信号通路可能参与UC疾病过程。

Objective To study the difference in gene expression and biological functions of intestinal macrophages in ulcerative colitis(UC).Methods Seven UC patients and seven healthy controls who visited the VA San Diego Healthcare facility from January to December 2019 were selected as the study subjects.The colonic mucosal single cell sequencing data were analyzed,and the differentially expressed genes in macrophages were compared,then gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis were performed.Results The cells were filtered and the cluster of macrophage was determined.A total of 31 differentially expressed genes were selected in intestinal mucosa of UC group and control group,including TIMP1,IFITM3,IGHG1,etc.GO and KEGG enrichment analysis of differentially expressed genes showed mainly enriched in the biological process of oxidative stress and IL-17 signaling pathway.Conclusion The oxidative stress and IL-17 signaling pathway of intestinal macrophages may be involved in the process of UC disease.