骨桥蛋白对胃癌细胞增殖、侵袭和迁移的影响

Effect of osteopontin on proliferation,invasion and migration of gastric cancer cells

目的探讨骨桥蛋白(OPN)对胃癌SGC-7901细胞增殖、侵袭和迁移能力的影响。方法体外培养胃癌SGC-7901细胞后分为control组、NC-shRNA组、OPN-shRNA组。MTT法检测细胞增殖;Transwell实验检测细胞的侵袭和迁移能力;Western blot实验检测细胞周期蛋白(cyclin D1)、血管内皮生长因子(VEGF)和基质金属蛋白酶2(MMP-2)蛋白表达水平。结果control组与NC-shRNA组细胞增殖、侵袭、迁移能力和相关蛋白表达水平比较,差异无统计学意义(P>0.05);在沉默OPN表达后,OPN-shRNA组细胞增殖、侵袭和迁移能力低于control组,差异有高度统计学意义(P<0.01);OPN-shRNA组cyclin D1、VEGF和MMP-2蛋白表达水平低于control组,差异有统计学意义(P<0.05)。结论OPN能增强胃癌SGC-7901细胞的增殖、侵袭和迁移能力,其作用机制可能与调节cyclin D1、VEGF和MMP-2的表达有关。

Objective To investigate the effect of osteopontin(OPN)on the proliferation,invasion,and migration ability of gastric cancer SGC-7901 cells.Methods Gastric cancer SGC-7901 cells were cultured in vitro and they were divided into three groups:control group,NC-shRNA group,OPN-shRNA group.The proliferation of cell was detected by MTT assay,and the invasion and migration were detected by Transwell assay.The expression of cyclin D1,vascular endothelial growth factor(VEGF),and matrix metallo proteinase 2(MMP-2)was detected by Western blot assay.Results There was no significant difference between control group and NC-shRNA group in cell proliferation,invasion,migration,and protein expression levels(P>0.05).After silencing OPN gene expression,OPN-shRNA had significant cell proliferation,invasion,and migration capabilities were lower than control group,the differences were highly statistically significant(P<0.01);the expression levels of OPN-shRNA cyclin D1,VEGF,and MMP-2 protein were lower than control group,and the difference was statistically significant(P<0.05).Conclusion OPN can enhance the proliferation,invasion,and migration of gastric cancer SGC-7901 cells,and its mechanism may be related to the regulation of cyclin D1,VEGF,and MMP-2 expression.