毛菊苣根-毛菊苣子配伍的化学成分研究

Study on the Chemical Constituents of Compatibility of Root and Seed of Cichorium Glandulosum

目的通过对毛菊苣根与毛菊苣子配伍前后化学成分进行定量定性分析,阐明二者配伍后化学成分变化。方法对毛菊苣根、毛菊苣子以及毛菊苣根-毛菊苣子3种配比(1∶2,1∶1,2∶1)的5个样品超声提取30 min;以菊苣酸为外标,采用HPLC测定各样品中18种化学成分的相对含量,并采用UPLC-TQ-MS分析化合物分子量信息、多级质谱裂解碎片的种类等信息,对配伍前后的特征化学成分进行定性鉴定。结果配伍前后样品中各化学成分的种类和含量变化较大,提示配伍后化合物发生化学变化;推测出样品中可能的16种化合物,其中秦皮甲素、壬二酸和5,8,3′,4′-四羟基-7-甲氧基黄酮为毛菊苣根与毛菊苣子的共有成分,菊苣酸和槲皮素-3-O-葡萄糖苷在配伍后消失,绿原酸和秦皮乙素则是配伍后新出现的化合物。结论毛菊苣根和毛菊苣子配伍使用后化学成分发生量变和质变,为其配伍机制阐明奠定基础。

OBJECTIVE To clarify the possible mechanism of the compatibility of the root and seed of Cichorium glandulosum.through quantitative and qualitative analysis of the chemical composition before and after compatible.METHODS Ultrasonic extraction method was used to extract 5 samples with the root of Cichorium glandulosum,the seeds of Cichorium glandulosum,and their three ratios(1∶2,1∶1,2∶1)for 30 min.Using chicory acid as the external standard,the relative contents of 18 chemical components in each sample were determined by HPLC.By analyzing the molecular weight of the compounds and multistage mass spectrometry fragmentation information provided by UPLC-TQ-MS to identify the characteristic chemical components.RESULTS The types and contents of the chemical components in the samples were changed greatly before and after compatibility,suggesting that the structure of compounds changed after compatibility.Structures of 16 components in five samples were preliminarily speculated by MS spectra.Among them esculine,azelaic acid and 5,8,3′,4′-tetrahydroxy-7-methoxylatedflavones were the common components of root and seed of Cichorium glandulosum,chicory acid and quercetin-3-O-glucoside disappeared after compatibility,and chlorogenic acid and esculetin were new compounds after compatibility.CONCLUSION Quantitative and qualitative changes lays a foundation for clarifying compatibility mechanism of the root and seed of Cichorium glandulosum.