UPLC同时测定不同产地三叶青不同部位中的7种活性成分

Simultaneous Determination of 7 Active Ingredients from Different Parts of Tetrastigma Hemsleyanum of Different Origins by UPLC

目的建立UPLC同时测定不同产地三叶青不同部位中7种活性成分含量。方法采用UPLC分离检测,色谱柱为BEH Shield RP18(150 mm×2.1 mm,1.7μm);柱温35℃;进样量2μL^(-1)流动相:乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱;流速0.3 mL·min^(-1);检测波长为360 nm和280 nm,采用外标法测定7种活性成分的含量。结果儿茶素、原花青素B1、芦丁、异槲皮素、山奈酚-3-O-芸香糖苷、紫云英苷、芹菜素分别在1.02~40.8,1.43~57.0,1.16~46.2,1.09~43.6,1.12~44.6,1.18~47.2,0.30~11.8μg·mL^(-1)呈良好的线性关系。不同产地三叶青和不同部位三叶青中各成分含量均有一定差异。结论采用UPLC同时测定三叶青中7种活性成分准确可行,为三叶青药材的质量控制提供了参考。

OBJECTIVE To establish a UPLC method for the simultaneous determination of 7 active ingredients in different parts of Tetrastigma hemsleyanum of different origins.METHODS UPLC was used for separation and detection.The chromatographic analysis was performed on BEH Shield RP18 column(150 mm×2.1 mm,1.7μm).The column temperature was 35℃.The injection volume was 2μL.The mobile phase was acetonitrile(A)-0.1% phosphoric acid solution(B)at the flow rate of 0.3 mL·min^(-1) with gradient elution.The detection wavelengths were 360 nm and 280 nm.The contents of 7 kinds of active ingredients were determined by external standard method.RESULTS Catechin,procyanidin B1,rutin,isoquercetin,kaempferol-3-O-rutoside,astragaloside and apigenin showed a good linear relationship in 1.02-40.8,1.43-57.0,1.16-46.2,1.09-43.6,1.12-44.6,1.18-47.2,0.30-11.8μg·mL^(-1),respectively.There were some differences in the contents of various componennts in different areas and different parts of T.Hesleyanur.CONCLUSION The established UPLC method for the simultaneous determination of 7 active ingredients in Tetrastigma hemsleyanum is accurate and feasible,which provides a reference for the quality control of Tetrastigmae Radix.