川续断组织培养外植体预处理方法研究

Study on Disinfection Method of Tissue Culture Explants of Dipsacus Asperoides

目的筛选川续断组织培养外植体预处理的最佳方式。方法先用单因素试验确定川续断叶片不同预处理方式(流水冲洗、75%酒精和0.1%升汞消毒)的最佳时间,在单因素试验的基础上,采用响应面优化法,对不同预处理方式进行优化。结果川续断愈伤诱导的最佳预处理方式为:流水冲洗1.02 h,75%酒精10.41 s,0.1%升汞15.72 min,在最佳预处理方式下,愈伤组织诱导率为95.32%。结论该研究筛选的川续断组织培养外植体最佳预处理方式,可为今后建立高效的川续断快繁体系提供基础资料。

Objective To screen the best way of explants pretreatment in Dipsacus asperoides tissue culture.Methods Firstly,the best time of different pretreatment methods(water washing,75%alcohol and 0.1%mercuric chloride disinfection)of Dipsacus asperoides leaves was determined by single factor test.On the basis of single factor test,response surface optimization method was used to optimize different pretreatment methods.Results The best pretreatment for callus induction of Dipsacus asperoides was as follows:water washing for 1.02 h,75% alcohol for 10.41 s,0.1% mercuric chloride for 15.72 min.Under the optimal pretreatment method,the callus induction rate was 95.32%.Conclusion The best pretreatment method of explants selected in this study can provide basic data for establishing efficient rapid propagation system of Dipsacus asperoides.