恒温扩增芯片法在下呼吸道感染病原体检测中的应用

Application of constant temperature amplification chip method in pathogens detection of lower respiratory tract infection

目的评价恒温扩增芯片法在下呼吸道感染病原体检测中的应用价值。方法选取四川大学华西医院2021年1—6月下呼吸道疑似感染患者1432例作为研究对象,采用恒温扩增芯片法检测感染病原体阳性率并与细菌传统培养法检测结果进行比较分析。结果研究对象采用恒温扩增芯片法检测出阳性例数为597例(阳性率为41.7%),采用细菌传统培养法检测出阳性例数为530例(阳性率为37.0%)。两种方法对铜绿假单胞菌检测一致性高(Kappa≥0.75),对流感嗜血杆菌、大肠埃希菌、肺炎链球菌及嗜麦芽窄食单胞菌检测一致性低(Kappa<0.40)。恒温扩增芯片法对鲍曼不动杆菌、肺炎克雷伯菌、铜绿假单胞菌及金黄色葡萄球菌等病原体的混合阳性率高于细菌传统培养法,差异有统计学意义(P<0.05)。结论恒温扩增芯片法检测下呼吸道感染病原体的阳性率高于细菌传统培养法检测的阳性率,有利于快速找到下呼吸道感染的真正病原体。

Objective To evaluate the value of constant temperature amplification chip method in detecting pathogens of lower respiratory tract infection.Methods A total of 1432 patients with suspected lower respiratory tract infection from January to June 2021 in West China Hospital,Sichuan University were selected as subjects.The positive rate of infection pathogens was detected by constant temperature amplification chip method and compared with the results of traditional bacterial culture method.Results 597 cases(positive rate 41.7%)were detected by constant temperature amplification chip method,530 cases(positive rate 37.0%)were detected by traditional bacterial culture method.The two methods had high consistency for Pseudomonas aeruginosa(Kappa≥0.75),but low consistency for Haemophilus influenzae,Escherichia coli,Streptococcus pneumoniae and Stenotrophomonas maltophilia(Kappa<0.40).The mixed positive rate of Acinetobacter baumannii,Klebsiella pneumoniae,Pseudomonas aeruginosa and Staphylococcus aureus by constant temperature amplification chip method was higher than that by traditional bacterial culture method,and the difference was statistically significant(P<0.05).Conclusion The positive rate of pathogens in lower respiratory tract infection detected by constant temperature amplification chip method is higher than that detected by traditional bacterial culture method,which is beneficial to quickly find the real pathogens of lower respiratory tract infection.