基于铁调素调控铁代谢相关蛋白探讨脑泰方干预氧糖剥夺损伤的神经元保护机制

Mechanism of Naotaifang intervention on oxygen-glucose deprivation injury of neurons based on hepcidin regulating iron metabolism related proteins

目的 观察大鼠原代神经元氧糖剥夺损伤后,脑泰方通过调节二价金属离子转运体1(divalent metal transporter 1,DMT1)、转铁蛋白受体(transferrin receptor,TfR)、铁调素(hepcidin,Hep)及膜铁转运蛋白(ferroportin,FPN)表达变化,探讨脑泰方对氧糖剥夺损伤神经元保护机制。方法 将原代培养大鼠神经元随机分为对照(CG)组、氧糖剥夺模型(OGD)组、脑泰方干预氧糖剥夺模型(NTF)组。MTT法检测各组神经元细胞存活率;荧光探针检测各组神经元细胞内二价铁离子(Fe^(2+))、活性氧(reactive oxygen species,ROS)浓度;Western blot法检测各组神经元细胞Hep、DMT1、TfR、FPN蛋白表达。结果 与CG组比较,OGD组、NTF组神经元细胞存活率均明显降低(P<0.01);与OGD组比较,NTF组神经元细胞存活率明显升高(P<0.01)。与CG组比较,OGD组、NTF组神经元细胞内Fe^(2+)、ROS浓度均明显升高(P<0.01);与OGD组比较,NTF组神经元细胞内Fe^(2+)、ROS浓度均显著降低(P<0.01)。与CG组比较,OGD组、NTF组神经元细胞Hep表达均明显降低(P<0.01),DMT1、TfR、FPN表达均明显升高(P<0.01);与OGD组比较,NTF组神经元细胞Hep表达明显升高(P<0.01),DMT1、TfR、FPN表达均明显降低(P<0.01,P<0.05)。结论 脑泰方通过促进神经元细胞Hep的表达,抑制神经元细胞DMT1、TfR、FPN表达,减少氧糖剥夺损伤后神经元细胞内Fe^(2+)及ROS聚积,起到保护神经元,减轻氧糖剥夺损伤的作用。

Objective To explore the protective mechanism of Naotaifang on neurons by observing the expression changes of divalent metal transporter 1(DMT1),transferrin receptor(TfR),hecpidin(Hep) and membrane iron transporter ferroportin(FPN)after oxygen-glucose deprivation injury in primary neuronal.Methods The rat neurons were maturely cultured and then randomly divided into the control(CG) group,the oxygen-glucose deprivation model(OGD) group and Naotaifang interventing the oxygen-glucose deprivation(NTF) group.Cell viability was measured by MTT assay in each group.The intracellular concentration of ferrous iron(Fe^(2+)) and reactive oxygen species(ROS) in neurons of each group were detected by fluorescent probe.The expression of Hep,DMT1,TfR,FPN were analyzed by Western blot.Results Compared with the CG group,the survival rate of neurons in OGD group and NTF group was significantly lower(P<0.01);compared with OGD group,the survival rate of neurons in NTF group was significantly higher(P<0.01).Compared with CG group,the concentrations of Fe^(2+) and ROS in neurons of OGD group and NTF group were significantly increased(P<0.01);compared with OGD group,the concentrations of Fe^(2+) and ROS in neurons of NTF group were significantly decreased(P<0.01).Compared with CG group,the expression levels of Hep in neurons of OGD group and NTF group were significantly decreased(P <0.01),while the expression levels of DMT1,Tf R and FPN were significantly increased(P<0.01);compared with OGD group,the expression of Hep in neurons of NTF group was significantly increased(P<0.01),while the expression levels of DMT1,Tf R and FPN were significantly decreased(P<0.01,P<0.05).Conclusion Naotaifang can protect neurons and reduce damage of oxygen-glucose deprivation injury by increasing the expression of Hep,inhibiting the expression of DMT1,Tf R and FPN,reducing the accumulation of Fe^(2+) and ROS in neurons after oxygen-glucose deprivation injury.