高效液相色谱法测定慢性胃炎颗粒中芍药苷的含量

Determination of Paeoniflorin in Chronic Gastritis Granules by HPLC

目的:建立慢性胃炎颗粒中芍药苷的含量测定方法。方法:采用高效液相色谱法,用十八烷基硅烷键合硅胶为填充剂;以乙腈为流动相A,以0.1%磷酸溶液为流动相B,检测波长为230 nm,柱温30 ℃,理论板数按芍药苷峰计算均大于2 000。结果:芍药苷在0.066 24~0.662 40 μg范围内具有良好线性关系。回归方程:Y=1 488.4X+7.751 5,r=0.999 9。平均回收率为100.57%,RSD为1.60%。结论:所建方法对慢性胃炎颗粒中的芍药苷进行定量,方法简便,灵敏,重现性好,可有效控制慢性胃炎颗粒制剂质量。

Objective:To establish a method for the determination of paeoniflorin in chronic gastritis granules.Methods:Using high performance liquid chromatography (HPLC),octadecylsilane bonded silica gel as filler;with acetonitrile as mobile phase A and 0.1% phosphoric acid solution as mobile phase B,the detection wavelength was 230 nm and the column temperature was 30 ℃.The theoretical plate number calculated according to paeoniflorin peak was more than 2 000.Results:The linear range of paeoniflorin was 0.066 24-0.662 40 μg.The regression equation:Y=1 488.4X+7.751 5,r=0.999 9.The average recovery was 100.57% and RSD was 1.60%.Conclusion:The established method for quantitative determination of paeoniflorin in chronic gastritis granules is simple,sensitive and reproducible,and can effectively control the quality of preparation of chronic gastritis granules.