miRNA-123介导mdm2-p53通路调节胃癌细胞增殖与凋亡的作用研究

miRNA-123 mediates MDM2-p53 pathway to regulate cell proliferation and apoptosis in gastric cancer

目的 探讨miRNA-123介导mdm2-p53通路调节胃癌细胞增殖与凋亡的作用。方法 选取对数生长期的CRL-5822细胞,随机分为miRNA-NC组、miRNA-123组和对照组,miRNA-NC组转染miRNA-123-mimic, miRNA-123组转染miRNA-NC,对照组行常规培养(不转染)。采用CCK-8法检测细胞增殖,Transwell小室试验检测细胞侵袭,流式细胞仪检测细胞凋亡,Western blot检测mdm2与p53蛋白表达。结果 转染后,miRNA-123组较miRNA-NC组和对照组,增殖指数和侵袭均偏低,差异均有统计学意义(均P<0.05);对照组和miRNA-NC组比较,差异无统计学意义(P>0.05)。miRNA-123组较miRNA-NC组和对照组,细胞凋亡指数偏高,差异均有统计学意义(均P<0.05);对照组和miRNA-NC组比较,差异无统计学意义(P>0.05)。细胞转染后24h与36h, miRNA-123组的mdm2蛋白相对表达水平低于对照组和miRNA-NC组,p53蛋白相对表达水平高于对照组和miRNA-NC组,差异均有统计学意义(均P<0.05);对照组和miRNA-NC组的mdm2和p53蛋白相对表达水平比较,差异无统计学意义(P>0.05)。结论 胃癌组织中的miRNA-123过表达,可抑制mdm2蛋白表达,促进p53蛋白表达,其作用机制与mdm2-p53通路调节有关,从而促进细胞凋亡,抑制胃癌细胞增殖与侵袭。

Objective To investigate the role of miRNA-123 mediated MDM2-p53 pathway in regulating the proliferation and apoptosis of gastric cancer cells. Methods CRL-5822 cells in logarithmic growth phase were selected and randomly divided into a miRNA-NC group, a miRNA-123 group and a control group. The miRNA-NC group and miRNA-123 group were transfected with miRNA-123-mimic and miRNA-NC, respectively and the control group were routinely cultured without transfection. CCK-8 method was used to detect cell proliferation, Transwell chamber assay was used to detect cell invasion, flow cytometry was used to detect cell apoptosis, and Western blot was used to detect the expression of MDM2 and p53 protein. Results After cell transfection, the proliferation index and invasion index were lower in the miRNA-123 group than in the miRNA-NC group and the control group(all P<0.05). However, there was no significant difference in the cell proliferation index and invasion index between the miRNA-NC group and the control group(P>0.05). The apoptosis indexes were relative were higher in the miRNA-123 group than in the miRNA-NC group and the control group(all P<0.05), and there was no significant difference between the control group and the miRNA-NC group(P>0.05). At 24 h and 36 h after the transfection, the expression of MDM2 was relatively lower in the miRNA-123 group than in the miRNA-NC group and the control group and the expression of p53 was relatively higher in the miRNA-123 group than in the miRNA-NC group and the control group(all P<0.05). However, there was no significant difference in the expression of MDM2 and p53 between the miRNA-NC group and the control group(P>0.05). Conclusion Overexpression of mirna-123 can inhibit the expression of MDM2 protein and promote the expression of p53 protein in gastric cancer. Its mechanism is associated with the regulation of MDM2-p53 pathway promoting apoptosis and inhibiting proliferation and invasion of gastric cancer cells.