基于网络药理学研究吴茱萸治疗结直肠癌作用机制

Study on the Mechanism of Evodia Rutaecarpa in Treating Colorectal Cancer based on Network Pharmacology

目的:应用网络药理学研究吴茱萸治疗结直肠癌的活性成分、作用靶点及机制。方法:通过基于中药系统药理学数据库(TCMSP)、GeneCards数据库查找吴茱萸活性成分,作用靶点及结直肠癌相关靶点,利用R语言(R 3.6.3)分析吴茱萸治疗结直肠癌的潜在作用靶点,并对关键靶点进行蛋白互作网络(PPI)分析以及基因本体(GO)和信息通路(KEGG)富集分析。结果:网络药理学研究获得吴茱萸治疗结直肠癌的潜在靶点162个。生物信息学分析吴茱萸通过PI3K-Akt,AGE-RAGE,MAPK,癌症中的蛋白多糖,细胞衰老,TNF,松弛素,细胞凋亡,HIF-1,雌激素,铂类耐药,EGFR酪氨酸激酶抑制剂抵抗,P53等信号通路发挥抗结直肠癌作用。结论:吴茱萸通过多靶点、多通路治疗结直肠癌,为吴茱萸的分子机制研究及临床应用提供了依据。

Objective To study the active components,target genes and mechanism of Evodia rutaecarpa against colorectal cancer based on network pharmacology.Methods The active components,potential targets and colorectal cancer-related targets of Evodia rutaecarpa were retrieved by TCMSP and GeneCards database.The potential targets of Evodia rutaecarpa in the treatment of colorectal cancer were analyzed by R language(R 3.6.3)and the key targets were analyzed by PPI analysis,GO and KEGG enrichment analysis.Results 162 potential targets of Evodia rutaecarpa in the treatment of colorectal cancer were obtained based on the study of network pharmacology.Bioinformatics analysis showed that Evodia rutaecarpa exerts its anti-colorectal cancer effect through PI3K-Akt signaling pathway,AGE-RAGE signaling pathway,MAPK signaling pathway,proteoglycans in cancer,cellular senescence,TNF signaling pathway,relaxin signaling pathway,apoptosis,HIF-1 signaling pathway,estrogen signaling pathway,platinum drug resistance,EGFR tyrosine kinase inhibitor resistance,P53 signaling pathway and et al.Conclusion Evodia rutaecarpa can treat colorectal cancer through multi-target and multi-pathway,which provides a basis for the study of molecular mechanism and clinical application of Evodia rutaecarpa.