蜜环菌菌丝与菌索活性成分含量研究

Study on the active ingredient content of mycelium and rhizomorphs of Armillaria mellea

目的测定蜜环菌(Armillariamellea)菌丝、菌索两种形态总多糖、总蛋白、总三萜、麦角甾酮、麦角甾醇、腺苷的含量差异。方法采用紫外-可见分光光度法测定总三萜、总多糖及总蛋白含量。采用高效液相色谱法测定麦角甾酮、麦角甾醇、腺苷成分含量。结果蜜环菌菌丝与菌索两种形态的大部分活性成分含量差异显著,其中总三萜、总蛋白、麦角甾醇成分显示菌丝含量优于菌索,且具有显著性差异(P<0.05);菌索总多糖含量、麦角甾酮含量优于菌丝,含量差异明显;两者腺苷含量相似。结论对于蜜环菌的药用以及开发保健食品方面,需要根据具体方案进行合理开发,达到资源的合理利用。研究结果可为今后的综合利用开发奠定理论基础。

Objective To study the differences in the content of Armillaria mellea mycelium and rhizomorphs,total polysaccharides,total proteins,total triterpenes,ergosterone,ergosterol and adenosine,provide a theoretical basis for the comprehensive development and utilization.Methods The content of total triterpenes,total polysaccharides and total protein was determined by ultraviolet-visible spectrophotometry.The content of ergosterone,ergosterol and adenosine was determined by high performance liquid chromatography.Results There were significant differences in the content of most active components between Armillaria mellea mycelium and rhizomorphs.The total triterpenes,total protein and ergosterol showed that the content of mycelium was better than that of rhizomorphs,and there was a significant difference(P<0.05);the total polysaccharides content and ergosterone content of rhizomorphs were better than mycelium,and the content difference was obvious;the content of adenosine was similar between the two.Conclusion For the medicinal use and development of Armillaria mellea and the development of health food,it is necessary to develop rationally according to specific plans to achieve rational utilization of resources.The research results can lay a theoretical foundation for future comprehensive utilization and development.