复方蛇蚁喷雾剂微生物限度检查方法的建立

Establishment of Microbial Limit Test Methods for Compound Snake-ant Spray

目的:建立复方蛇蚁喷雾剂的微生物限度检查方法。方法:选用中山市黄圃人民医院制剂中心自2017年11月至2017年12月间配制的复方蛇蚁喷雾剂,以试验菌的回收比为指标,采用平皿法、薄膜过滤法考察需氧菌总数、霉菌和酵母菌总数的计数方法;以供试品中的金黄色葡萄球菌、铜绿假单胞菌的有效检出为指标,采用平皿法、薄膜过滤法考察控制菌检查法。结果:需氧菌总数计数方法采用薄膜过滤法、霉菌和酵母菌总数计数方法采用平皿法时,各试验组菌落数与菌液对照组菌落数的比值均在0.5~2.0。控制菌检查法采用薄膜过滤法时,能检出金黄色葡萄球菌、铜绿假单胞菌。结论:本研究建立的方法适用于复方蛇蚁喷雾剂的微生物限度检查。

Objective To establish a microbial limit test method for Compound snake-ant spray.Methods The compound the compound snake-ant spray by Huangpu People's Hospital of Zhongshan from November 2017 to December 2017 was selected.The recovery ratio of the test bacteria was as an index.The total number of aerobic bacteria,fungus and yeast were counted by plate method and membrane filtration method.The effective detection of staphylococcus aureus and pseudomonas aeruginosa in the tested samples was taken as the index,and the control bacteria were examined by plate method and membrane filtration method.Results When the count method of aerobic bacteria was membrane filtration method and the count method of fungus and yeast was plate method,the ratio of colony number of experimental groups to that of liquid control group was between 0.5 and 2.0.Control bacteria inspection method using membrane filtration method,can detect staphylococcus aureus,pseudomonas aeruginosa.Conclusion The method established in this study is suitable for the microbial limit test of compound snake-ant spray.