摘要
Photoacoustic microscopy is an in vivo imaging technology based on the photoacoustic effect.It is widely used in various biomedical studies because it can provide high-resolution images while being label-free,safe,and harmless to biological tissue.Polygon-scanning is an effective scanning method in photoacoustic microscopy that can realize fast imaging of biological tissue with a large field of view.However,in polygon-scanning,fluctuations of the rotating motor speed and the geometric error of the rotating mirror cause image distortions,which seriously affect the photoacoustic-microscopy imaging quality.To improve the image quality of photoacoustic microscopy using polygon-scanning,an image correction method is proposed based on accurate ultrasound positioning.In this method,the photoacoustic and ultrasound imaging data of the sample are simultaneously obtained,and the angle information of each mirror used in the polygon-scanning is extracted from the ultrasonic data to correct the photoacoustic images.Experimental results show that the proposed method can significantly reduce image distortions in photoacoustic microscopy,with the image dislocation offset decreasing from 24.774 to 10.365μm.
基金
This work was supported by the National Natural Science Foundation of China
Nos.91739117 and 81927807
Shenzhen Science and Technology Innovation,No.JCYJ20170413153129570
Chinese Academy of Sciences Nos.YJKYYQ20190078 and GJJSTD20180002
Shenzhen Key Laboratory for Molecular Imaging,No.ZDSY20130401165820357
Guangdong Provincial Key Laboratory of Biomedical Optical Imaging,No.2020B121201010.
