前列腺癌组织中LncRNA LINC00355、miR-494-3p、CCND2的表达及临床意义

Expression and clinical significance of LncRNA LINC00355,miR-494-3p,and CCND2 mRNA in prostate cancer tissues

目的探讨前列腺癌组织中长链非编码RNA(LncRNA)LINC00355、微小RNA-494-3p(miRNA-494-3p)、细胞周期素D2(CCND2)的表达及临床意义。方法选取安徽省第二人民医院2015年1月至2017年12月收治的91例前列腺癌患者,qRT-PCR检测患者癌组织和癌旁正常组织中LncRNA LINC00355、miR-494-3p、CCND2 mRNA表达。Kaplan-Meier曲线分析不同LncRNA LINC00355、miR-494-3p、CCND2 mRNA表达前列腺癌患者的术后3年生存率。结果前列腺癌组织中LncRNA LINC00355、CCND2 mRNA表达高于癌旁正常组织,miR-494-3p表达低于癌旁正常组织(P<0.05)。前列腺癌组织中LncRNA LINC00355与miR-494-3p表达呈负相关,与CCND2 mRNA表达呈正相关(r=-0.502、0.458,P<0.001),miR-494-3p与CCND2 mRNA表达呈负相关(r=-0.493,P<0.001)。LncRNA LINC00355高表达、miR-494-3p低表达、CCND2 mRNA高表达患者术后3年总生存率低于LncRNA LINC00355低表达、miR-494-3p高表达、CCND2 mRNA低表达患者(P<0.05)。结论前列腺癌组织中LncRNA LINC00355、CCND2表达上调,miR-494-3p表达下调,LncRNA LINC00355可能通过抑制miR-494-3p上调CCND2表达,促进前列腺癌进展。

Objective To investigate the expression and clinical significance of long non-coding RNA(LncRNA)LINC00355,microRNA-494-3p(miRNA-494-3p),cyclin D2(CCND2)mRNA expression in prostate cancer tissues.Methods A total of 91 prostate cancer patients admitted to Anhui NO.2 Provincial People’s Hospital from January 2015 to December 2017 were selected.qRT-PCR was used to detect the expression of LncRNA LINC00355,miR-494-3p,and CCND2 mRNA in cancer tissues and paracancer normal tissues of patients.Kaplan-Meier curve was used to analyze three-year survival rate after surgery of prostate cancers with different LncRNA LINC00355,miR-494-3p,and CCND2 mRNA expression.Results LncRNA LINC00355 and CCND2 mRNA expression in prostate cancer tissues were higher than those in paracancer normal tissues,and miR-494-3p expression was lower than that in paracancer normal tissues(P<0.05).LncRNA LINC00355 was negatively correlated with miR-494-3p expression and positively correlated with CCND2 mRNA expression in prostate cancer tissues(r=-0.502,0.458,P<0.001),and miR-494-3p was negatively correlated with CCND2 mRNA expression(r=-0.493,P<0.001).The three-year overall survival rate after surgery in patients with high LncRNA LINC00355 expression,low miR-494-3p expression,and high CCND2 mRNA expression were lower than those in patients with low LncRNA LINC00355 expression,high miR-494-3p expression,and low CCND2 mRNA expression(P<0.05).Conclusion LncRNA LINC00355 and CCND2 expression are up-regulated and miR-494-3p expression is down-regulated in prostate cancer tissues.LncRNA LINC00355 may promote prostate cancer progression by inhibiting miR-494-3p and up-regulating CCND2 expression.