吸入高浓度H_(2)对高脂饮食大鼠血浆和肝脏中磷脂和鞘脂分子的影响

Effects of high concentration of H_(2) inhalation on phospholipids and sphingolipids in plasma and liver of high-fat diet rats

目的H_(2)吸入对脂质代谢有一定的调节作用,但对磷脂、鞘脂分子的具体影响尚无研究。本研究通过靶向脂质组学方法检测了66.7%的H_(2)吸入干预后高脂饮食大鼠血浆及肝脏中的磷脂、鞘脂分子的变化,为研究H_(2)改善脂代谢的机制研究提供依据。方法选取雄性SD大鼠分为对照组、高脂饮食模型组,高脂饮食+66.7%H_(2)干预组。干预10周后,对血浆和肝脏中的不同种类的磷脂和鞘脂进行分析。结果主成分分析及聚类热图表明高脂饮食模型组与对照组之间的磷脂、鞘脂谱存在明显差异,在血浆中检测出差异磷脂分子17种,差异鞘脂分子16种;在肝脏中检测出差异磷脂分子50种,鞘脂分子18种。66.7%H_(2)干预后对血浆的磷脂和鞘脂均有影响,共计5种磷脂酰胆碱、2种溶血磷脂酰乙醇胺、1种鞘磷脂分子含量降低,差异有统计学意义(P<0.05);66.7%H_(2)干预后对肝脏磷脂影响较大,共12种磷脂酰胆碱、6种磷脂酰乙醇胺、3种溶血磷脂酰胆碱、4种溶血磷脂酰乙醇胺分子含量降低,差异有统计学意义(P<0.05),而对神经酰胺、鞘磷脂等鞘脂无显著影响。结论66.7%的H_(2)吸入可影响高脂饮食大鼠磷脂、鞘脂代谢,降低血浆及肝脏中多种磷脂的含量,改善高脂饮食导致的血浆、肝脏中多种磷脂、鞘脂分子水平的异常。H_(2)可作为一种改善脂质代谢的有效辅助手段。H_(2)改善脂质代谢的分子机制值得进一步研究。

Objective:Hydrogen inhalation has a certain regulatory effect on lipid metabolism,but the specific effects on phospholipids and sphingolipids are not yet studied.In this article,the changes of phospholipids and sphingolipids were detected by targeted lipidomics method after inhalation of 66.7% hydrogen gas.Methods:Male SD rats were divided into control group,high-fat diet model group and high-fat diet+66.7% H_(2) intervention group.After 10 weeks of intervention,different kinds of phospholipids and sphingolipids in plasma and liver of the rats were analyzed.Results:Targeted lipidomics analysis showed differential hepatic and plasmatic effects of control and modern group,including 17 differential phospholipids and 16 differential sphingolipids in plasma,and 50 differential phospholipids and 18 differential sphingolipids liver.Phospholipids and sphingolipids in plasma and liver were significantly changed after 66.7% hydrogen intervention,including 5 phosphatidylcholines,2 lysophosphatidylethanolamines and 1 sphingomyelin decreased significantly in plasma;12 phosphatidylcholines,6 phosphatidylethanolamines,3 lysophosphatidylcholines,and 4 lysophosphatidyl ethanolamines decreased in liver,but there was no significant effects on ceramides and sphingomyelin sphingolipids.Conclusion:66.7% hydrogen inhalation can affect the metabolism of phospholipids and sphingolipids,and reduce the content of phospholipids,improve the abnormal levels of phospholipids and sphingolipids in plasma and liver of high-fat diet rats.Hydrogen may be an auxiliary means to improve lipid metabolism.The molecular mechanism of hydrogen improving lipid metabolism deserves further study.