血清TSH MPO铁蛋白在甲状腺功能减退症中的表达及相关性

Expression and Correlation of Serum TSH MPO and Ferritin in Hypothyroidism

目的:探究血清促甲状腺素(TSH)、髓过氧化物酶(MPO)、铁蛋白在甲减中的表达及相关性。方法:选取2019年5月至2020年5月我院收治的337例甲状腺功能减退症患者为观察组,同期选取350例甲状腺功能正常者为对照组,采用罗氏E601全自动电化学发光免疫分析仪及其配套试剂盒测定TSH、铁蛋白水平,采用双抗体夹心ELISA法测定两组血清MPO表达,分析TSH、MPO、铁蛋白在甲减患者中的表达及相关性。结果:相比对照组,观察组患者血清TSH、MPO升高,铁蛋白、游离三碘甲腺原氨酸(Free triiodothyronine;FT3)、游离甲状腺素(Free thyroxine;FT4)水平降低(P<0.05);以TSH、MPO、铁蛋白表达进行相关性分析,TSH、MPO呈正相关(r=0.458/P=0.001),TSH、铁蛋白呈负相关(r=-0.368/P=0.001),MPO、铁蛋白呈负相关(r=-0.343/P=0.001);TSH、MPO与甲减患者甲状腺激素(FT3、FT4)变化呈负相关,铁蛋白与甲减患者甲状腺激素变化呈正相关。将TSH、MPO、铁蛋白纳入二分类多因素Logistic回归模型分析,结果显示,TSH、MPO为影响甲减发生的危险因素,铁蛋白为影响甲减发生的保护因素(P<0.05)。结论:血清TSH、MPO在甲减中呈异常高表达,铁蛋白呈异常低表达,参与疾病演进,对其进行检测,能够为临床甲减患者诊断及治疗提供一定的参考依据。

Objective:To investigate the expression and correlation of serum thyrotropin(TSH),myeloperoxidase(MPO)and ferritin in hypothyroidism.Methods:From May 2019 to May 2020,337 patients with hypothyroidism admitted to our hospital were selected as the observation group,and 350 patients with normal thyroid function were selected as the control group during the same period.TSH and ferritin levels were measured by Roche E601 automatic electrochemiluminescence immunoassay analyzer and its supporting kit,and serum MPO expression was measured by double antibody sandwich ELISA in both groups to analyze the expression and correlation of TSH,MPO and ferritin in hypothyroid patients.Results:Compared to the control group,serum TSH and MPO were increased and ferritin,free triiodothyronine(FT3)and free thyroxine(FT4)levels were decreased in the observation group(P<0.05);correlation analysis was performed with TSH,MPO and ferritin expression.TSH and MPO were positively correlated(r=0.458/P=0.001),TSH and ferritin were negatively correlated(r=-0.368/P=0.001),MPO and ferritin were negatively correlated(r=-0.343/P=0.001);TSH and MPO were negatively correlated with changes in thyroid hormones(FT3 and FT4)in hypothyroid patients,while ferritin was positively correlated with changes in thyroid hormones in hypothyroid patients.TSH,MPO and ferritin were included in a dichotomous multi-factor logistic regression model analysis,and the results showed that TSH and MPO were risk factors and ferritin was a protective factor for the development of hypothyroidism(P<0.05).Conclusion:Serum TSH and MPO are abnormally high in hypothyroidism,and ferritin is abnormally low in hypothyroidism,which are involved in the evolution of the disease,and their detection can provide a certain reference for the diagnosis and treatment of clinical hypothyroid patients.