一株产纤维素酶降香内生真菌的分离鉴定及产酶活性检测

Separation and Identification of Cellulase-Producing Endophytic Fungi from Dalbergia odorifera and Detection of Enzyme-Producing Activity

从降香中分离纯化获得一株产纤维素酶内生真菌JXP2-2,并对其进行了产酶活性测定和分子生物学鉴定。结果表明,菌株JXP2-2在PDB液体培养基中培养4.5 d时的产酶活性最强,纤维素酶活力达到84.50 IU·mL^(-1);菌株JXP2-2与尖孢炭疽菌(Colletotrichum acutatum)KX347475的碱基相似度高达99%,初步鉴定其为尖孢炭疽菌。

We isolated and purified a cellulase-producing endophytic fungi JXP2-2 from Dalbergia odorifera,detected the enzyme-producing activity of the strain JXP2-2,and identified it by molecular biology.The results show that the strain JXP2-2 has the strongest enzyme-producing activity when cultured in PDB liquid medium for 4.5 d,and the cellulase activity reaches 84.50 IU·mL^(-1).The similarity of base between the strain JXP2-2 and Colletotrichum acutatum KX347475 is up to 99%,and it is preliminarily identified as Colletotrichum acutatum.