基质辅助激光解吸电离飞行时间质谱对羊源多杀性巴氏杆菌的快速鉴定

Rapid Identification of Sheep-Originated Pasteurella multocida by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

本试验通过基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对临床分离的1株羊源多杀性巴氏杆菌进行了快速鉴定,取得了与VITEK2 Compact传统生化方法和16S rRNA测序方法一致的结果。MALDI-TOF MS对分离菌株的鉴定分数为2.371,达到了种水平的鉴定。从分离到纯菌落到获得有效的鉴定结果,MALDI-TOF MS仅需要约30 min,而VITEK2需要约4 h,16S rRNA测序方法需要至少2 d。因此,MALDI-TOF MS可以实现对临床分离的多杀性巴氏杆菌菌株的快速、准确鉴定,对进一步改善动物巴氏杆菌病的临床诊治具有重要意义。

This study utilized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) for rapid identification of a Pasteurella multocida isolate from the lung and liver of suddenly dead sheep. MALDI-TOF MS yielded an identification score of 2.371, and achieved accurate identification to the species level, which was in agreement to that by the VITEK2 compact-based classical biochemical analysis and the 16 S rRNA gene sequencing analysis. From clinical sample, to bacterial pure culture, to identification result, MALDI-TOF MS took approximately 30 min, VITEK2 compact required approximately 4 h, and 16 S rRNA gene sequencing analysis demanded at least 2 days. Thus, MALDI-TOF MS enables rapid and accurate identification of P. multocida, and provides a valuable means to further improve the diagnosis and management of animal pasteurellosis.