内源性SGK1抑制地塞米松诱导成骨细胞自噬的机制研究

Mechanism of Endigenous SGK1 Inhibiting Dexamethasone Induced Autophagy in Osteoblasts

目的探究内源性SGK1抑制地塞米松诱导成骨细胞自噬的机制。方法购买小鼠成骨细胞MC3T3-E1,设置分组siRNA-SGK1组、siRNA-NC组。观察转染效率、FOXO3、ULK1、p-FOXO3、p-ULK1、LC3a、Beclin-1、Lamp-1。结果siRNA-SGK1组LC3a、Beclin-1基因表达及FOXO3、ULK1和磷酸化水平明显低于siRNA-NC组,P<0.05。结论内源性SGK1可明显抑制地塞米松诱导的成骨细胞自噬过程。

Objective To explore the mechanism of endogenous SGK1 inhibiting dexamethasone-induced autophagy in osteoblasts.Method Purchase mouse osteoblast MC3 T3-E1,and set up the siRNA-SGK1 group and siRNA-NC group.Observe the transfection efficiency,FOXO3,ULK1,p-FOXO3,p-ULK1,LC3 a,Beclin-1,Lamp-1.Results LC3 a,Beclin-1 gene expression,FOXO3,ULK1 and phosphorylation levels in the siRNA-SGK1 group were significantly lower than those in the siRNA-NC group,P<0.05.Conclusion Endogenous SGK1 can significantly inhibit the autophagy process of osteoblasts induced by dexamethasone.