基于指纹图谱和多指标成分含量测定的枳壳与枳实药材质量差异性研究

Quality difference in Aurantii Fructus and Aurantii Fructus Immatutus based on fingerprint analysis and multi-index content determination

目的建立测定枳壳与枳实中多指标成分含量及超高效液相色谱(UPLC)指纹图谱的方法,为枳壳与枳实的质量评价提供依据。方法采用Agilent SB C_(18)(100 mm×2.1 mm,1.8μm)色谱柱,以乙腈-0.05%磷酸为流动相,梯度洗脱,流速0.4 mL·min^(-1),柱温30℃,检测波长320 nm,对18批枳壳、10批枳实的特征图谱及多个指标成分的含量进行测定,并运用相关软件进行分析。结果枳壳及枳实UPLC特征图谱中确定18个共有峰,聚类分析和主成分分析可将28个样品按枳壳与枳实聚为两类;同时对已归属的8个特征峰进行含量测定。结论本研究建立的方法简便、快捷、可靠,可为枳壳及枳实的质量评价提供参考。

Objective To determine multi-index content and chromatogram for Aurantii Fructus and Aurantii Fructus Immatutus by UPLC and to provide reference for their quality control.Methods Agilent SB C_(18) column(100 mm×2.1 mm,1.8μm)was used.The mobile phase consisted of acetonitrile-0.05%phosphoric acid as the mobile phase with gradient elution at the flow rate of 0.4 mL·min^(-1).The column temperature was 30℃and the detection wavelength was 320 nm.Totally 18 batches of Aurantii Fructus and 10 batches of Aurantii Fructus Immatutus were determined and analyzed with related softwares.Results Eighteen common peaks were identified in the UPLC spectra of Aurantii Fructus and Aurantii Fructus Immatutus.Cluster analysis and principal component analysis clustered 28 samples into 2 groups according to Aurantii Fructus and Aurantii Fructus Immatutus.At the same time,the content of 8 characteristic peaks was determined.Conclusion The method is simple,rapid and reliable,providing reference for the quality evaluation of Aurantii Fructus and Aurantii Fructus Immatutus.