宁夏银川地区番茄斑萎病毒和南方番茄病毒复合侵染分子鉴定

Molecular Identification of Co-infection of Tomato Spotted Wilt Virus and Southern Tomato Virus in Yinchuan Region of Ningxia

2021年在宁夏银川地区病害调查时发现番茄植株茎秆和未成熟果实上出现了褐色、不规则形病斑等疑似病毒病的症状。采用Trizol法提取番茄病株样品的总RNA,使用常见侵染番茄的病毒特异性引物进行RT-PCR检测。结果显示:番茄斑萎病毒(tomato spotted wilt virus,TSWV)和南方番茄病毒(southern tomato virus,STV)的特异性引物分别从样品cDNA中扩增出777 bp和681 bp的目的产物,通过对产物测序并进行BLAST比对和系统发育分析,发现宁夏银川地区番茄上的TSWV与西班牙辣椒上的TSWV分离物聚在同一分支上,进化关系最近;STV分离物与法国、巴基斯坦、哥伦比亚番茄上的STV分离物的进化关系最近,与其他地区的STV分离物关系较远,表明宁夏银川地区的番茄被TSWV和STV复合侵染。

During disease investigation in Yinchuan region of Ningxia in 2021,we found suspected virus disease symptoms of brown and irregular spot on tomato plant stems and unmatured fruits.By Trizol method,we extracted the total RNA of infected tomato plant samples,and conducted RT-PCR detection using specific primers of common virus frequently infecting tomato.The results showed that the specific primers of tomato spotted wilt virus(TSWV)and southern tomato virus(STV)had amplified 777 bp and 681 bp target outcomes,respectively.Outcome sequencing,BLAST comparison conducting and Phylogenetic analysis showed that TSWV isolate from Yinchuan Region of Ningxia was on the same branch with TSWV isolate from Spanish pepper.Two of them had the closest relation.STV isolate had the closest evolutionary relation with STV isolates on tomatoes from France,Pakistan and Colombia,but had farther relation with STV isolates from the other regions.These results indicated that tomatoes in Yinchuan Region of Ningxia were co-infected by TSWV and STV.